ArticleParasites & vectors2019
Rapid multiplex gene expression assays for monitoring metabolic resistance in the major malaria vector Anopheles gambiae.
Article in Parasites & vectors, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- Trial
- Agricultural practices drive multifactorial pyrethroid resistance in Anopheles coluzzii populations from southern Benin.Tropical medicine and health · 2026Article
- Integrating Community Practices, Vector Resistance, and Molecular Xenomonitoring of Wuchereria Bancrofti: A One Health Implementation Study in Lymphatic Filariasis-Endemic Communities.Acta parasitologica · 2026Article
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- The Ifakara Ambient Chamber Test (I-ACT) for Evaluation of Indoor Residual Sprays: A Non-Inferiority Test of SylandoInsects · 2026Article
- Metarhizium pingshaense infection reverses insecticide resistance in Anopheles gambiae sensu lato by altering energy reserves and gene expression.Parasites & vectors · 2025Article
- Insecticide resistance and malaria transmission indicators in Anopheles gambiae s.l. in Bobo-Dioulasso, Burkina Faso: implications for vector control strategies.Tropical medicine and health · 2025Article
- Exploring the metabolic and cuticular mechanisms of increased pyrethroid resistance in Anopheles gambiae S.l populations from Ghana.Scientific reports · 2025Article
- Article
- Transcriptome analysis of Drosophila suzukii reveals molecular mechanisms conferring pyrethroid and spinosad resistance.Scientific reports · 2024Article
- Bio-efficacy of field aged novel class of long-lasting insecticidal nets, against pyrethroid-resistant malaria vectors in Tanzania: A series of experimental hut trials.PLOS global public health · 2024Article
- Evaluation of diffuse reflectance spectroscopy for predicting age, species, and cuticular resistance of Anopheles gambiae s.l under laboratory conditions.Scientific reports · 2023Article
- Sub-lethal exposure to chlorfenapyr reduces the probability of developing Plasmodium falciparum parasites in surviving Anopheles mosquitoes.Parasites & vectors · 2023Article
- Genome-wide association studies reveal novel loci associated with pyrethroid and organophosphate resistance in Anopheles gambiae and Anopheles coluzzii.Nature communications · 2023Article
- Relationship between insecticide resistance profiles in Anopheles gambiae sensu lato and agricultural practices in Côte d'Ivoire.Parasites & vectors · 2023Article
- Advances in point-of-care genetic testing for personalized medicine applications.Biomicrofluidics · 2023Review
- Species composition and insecticide resistance in malaria vectors in Ellibou, southern Côte d'Ivoire and first finding of Anopheles arabiensis in Côte d'Ivoire.Malaria journal · 2023Article
- Genome-wide association studies reveal novel loci associated with pyrethroid and organophosphate resistance inbioRxiv : the preprint server for biology · 2023Article
- Phenotypic resistance to pyrethroid associated to metabolic mechanism inWellcome open research · 2023Article
- Genetic Diversity of Cytochrome P450sGenes · 2022Article
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6 authors.
Funding
Abstract
backgroundMetabolic resistance of the major malaria vector Anopheles gambiae (s.l.) to insecticides is operationally significant, particularly in combination with target site resistance. However, detection of metabolic resistance is not trivial and relies on laborious bioassays, unspecific biochemical methods, or sophisticated and expensive molecular approaches using transcriptomics.
methodsRapid one-step multiplex TaqMan-probe based RT-qPCR assays were developed and optimised to measure the expression levels of genes associated with metabolic insecticide resistance in An. gambiae (s.l.). Primers and probes were designed to target the mRNA of cytochrome P450-dependent monooxygenases CYP6P3, CYP6M2, CYP9K1, CYP6P4 and CYP6Z1, and the glutathione-S-transferase GSTE2. The novel assays were validated versus gold standard methods with a range of phenotyped mosquito specimens. The assays were also tested directly on lysates of RNAlater®-preserved mosquitoes without an RNA extraction step.
resultsThe novel assays are efficient (reaction efficiencies = 95-109%), sensitive (covering a > 10.0 Ct range with R
conclusionsThe novel multiplex assays for monitoring the levels of major detoxification genes and metabolic resistance in An. gambiae (s.l.) are simple to perform, robust and rapid. They may complement current diagnostic assays to provide evidence-based and operationally relevant information for insecticide resistance management.
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