Evidence map›Paper›PMID 30611434›Full record

ArticleMethods in enzymology2019

A Quick and Colorful Method to Measure Low-Level Contaminations of Paramagnetic Ni

Starla D Glover, Cecilia Tommos

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in enzymology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Hydrogen production by a fullyDalton transactions (Cambridge, England : 2003) · 2024
    Article
  2. A Novel D-π-A Type Fluorescent Probe for CuJournal of fluorescence · 2023
    Article
  3. Article
  4. The effect of an engineered ATCUN motif on the structure and biophysical properties of the SH3 domain of c-Src tyrosine kinase.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Starla D GloverDepartment of Biochemistry & Biophysics, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, United States.
Cecilia TommosDepartment of Biochemistry & Biophysics, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, United States. Electronic address: tommos@pennmedicine.upenn.edu.

Funding

Understanding Protein RadicalsR01GM079190 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI TOMMOS, CECILIA · 2008 to 2024
$3.6M
NIGMS NIH HHS R01 GM079190
6 · The paper itself

Abstract

Isotopic labeling of recombinantly expressed proteins is generally required for investigation by modern nuclear magnetic resonance (NMR) methods. Purification strategies of the labeled proteins often include the use of a polyhistidine affinity tag (His-tag) and immobilized metal ion affinity chromatography (IMAC). Described herein are rapid and inexpensive qualitative and quantitative assays to determine the concentration of paramagnetic Ni

Indexed as

Cations, DivalentChromatography, AffinityChromogenic CompoundsColorimetryCoordination ComplexesEscherichia coliGene ExpressionGenetic VectorsMagnetic Resonance SpectroscopyNickelRecombinant Fusion ProteinsResorcinolsSensitivity and SpecificityThioredoxins4-(2-pyridylazo)resorcinolCations, DivalentChromogenic CompoundsCoordination ComplexesNickelRecombinant Fusion ProteinsResorcinolsThioredoxins4-(2-Pyridylazo)resorcinolIMACImmobilized metal ion affinity chromatographyNi(2+) leakagePARProtein metal ion contaminantsPurification of His-tagged proteins

Identifiers

PMID30611434
PMCPMC7363030

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.