ArticleMethods in molecular biology (Clifton, N.J.)2019
An Automated Protoplast Transformation System.
Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Article
- Toward a monocot SynBio toolkit: assessing regulatory element performance and eudicot compatibility.Plant cell reports · 2025Article
- Enhancing lipid production in plant cells through automated high-throughput genome engineering and phenotyping.The Plant cell · 2025Article
- Protoplast Technology and Somatic Hybridisation in the Family Apiaceae.Plants (Basel, Switzerland) · 2023Review
- Building the Plant SynBio Toolbox through Combinatorial Analysis of DNA Regulatory Elements.ACS synthetic biology · 2022Article
- A cationic lipid mediated CRISPR/Cas9 technique for the production of stable genome edited citrus plants.Plant methods · 2022Article
- Will Plant Genome Editing Play a Decisive Role in "Quantum-Leap" Improvements in Crop Yield to Feed an Increasing Global Human Population?Plants (Basel, Switzerland) · 2021Review
- Advances and Perspectives of Transgenic Technology and Biotechnological Application in Forest Trees.Frontiers in plant science · 2021Review
- Development and validation of a novel and robust cell culture system in soybean (Glycine max (L.) Merr.) for promoter screening.Plant cell reports · 2019Article
- Embryogenic cell suspensions for high-capacity genetic transformation and regeneration of switchgrass (Biotechnology for biofuels · 2019Article
- The plastid genome as a chassis for synthetic biology-enabled metabolic engineering: players in gene expression.Plant cell reports · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Efficient plant protoplast production from cell suspension cultures, leaf, and stem tissue allows for single-cell plant biology. Since protoplasts do not have cell walls, they can be readily transformed to enable rapid assessment of regulatory elements, synthetic constructs, gene expression, and more recently genome-editing tools and approaches. Historically, enzymatic cell wall digestion has been both expensive and laborious. Protoplast production, transformation, and analysis of fluorescence have recently been automated using an integrated robotic system. Here we describe its use for bulk protoplast isolation, counting, transformation, and analysis at very low cost for high-throughput experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.