ArticleMethods in molecular biology (Clifton, N.J.)2019
Biolistic Delivery of CRISPR/Cas9 with Ribonucleoprotein Complex in Wheat.
Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Who cites it
14 citing papers in PubMed.
- Review
- Breeding for improved digestibility and processing of lignocellulosic biomass inFrontiers in plant science · 2024Review
- CRISPR/Cas9 Landscape: Current State and Future Perspectives.International journal of molecular sciences · 2023Review
- Gene Editing Profiles in 94 CRISPR-Cas9 Expressing TPlants (Basel, Switzerland) · 2022Article
- Tissue Culture and Somatic Embryogenesis in Warm-Season Grasses-Current Status and Its Applications: A Review.Plants (Basel, Switzerland) · 2022Review
- There and back again; historical perspective and future directions forHorticulture research · 2022Article
- Plant biomacromolecule delivery methods in the 21st century.Frontiers in genome editing · 2022Review
- Transgene-free genome editing and RNAi ectopic application in fruit trees: Potential and limitations.Frontiers in plant science · 2022Review
- Protocol for assessment of the efficiency of CRISPR/Cas RNP delivery to different types of target cells.PloS one · 2021Article
- Strategies in the delivery of Cas9 ribonucleoprotein for CRISPR/Cas9 genome editing.Theranostics · 2021Review
- Non-GM Genome Editing Approaches in Crops.Frontiers in genome editing · 2021Review
- How to start your monocot CRISPR/Cas project: plasmid design, efficiency detection, and offspring analysis.Rice (New York, N.Y.) · 2020Review
- Comparison of CRISPR-Cas9/Cas12a Ribonucleoprotein Complexes for Genome Editing Efficiency in the Rice Phytoene Desaturase (OsPDS) Gene.Rice (New York, N.Y.) · 2020Article
- High-frequency random DNA insertions upon co-delivery of CRISPR-Cas9 ribonucleoprotein and selectable marker plasmid in rice.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The great advances in exploiting the CRISPR/Cas9 system are paving the way for targeted genome engineering in plants. Genome editing by direct delivery of CRISPR/Cas9 ribonucleoprotein complexes (RNPs) into plant cells reduces off-target mutations and avoids the integration of foreign DNA fragments, thus providing an efficient and accurate method for precision crop breeding. Here we describe an RNP-based genome editing protocol for wheat. The protocol covers the in vitro transcription of sgRNA, purification of Cas9 protein, biolistic delivery of CRISPR/Cas9 RNPs, and tissue culture procedures for regenerating testable seedlings.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.