ArticleMethods in molecular biology (Clifton, N.J.)2019
An Agrobacterium-Mediated CRISPR/Cas9 Platform for Genome Editing in Maize.
Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The trial behind it
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Who cites it
6 citing papers in PubMed.
- CRISPR/Cas9-Mediated Editing in FAD2 Gene to Enhance Oil Quality in Soybean [Glycine max (L.) Merrill].PloS one · 2026Article
- Comparative transcriptome profiling and co-expression network analysis reveals important genes regulating maize response to Southern corn rust.BMC plant biology · 2025Article
- CRISPR-Cas technology opens a new era for the creation of novel maize germplasms.Frontiers in plant science · 2022Review
- CRISPR-Cas technology in corn: a new key to unlock genetic knowledge and create novel products.Molecular breeding : new strategies in plant improvement · 2021Article
- Improving Horticultural Crops via CRISPR/Cas9: Current Successes and Prospects.Plants (Basel, Switzerland) · 2020Review
- High-throughput phenotypic screening of random genomic fragments in transgenic rice identified novel drought tolerance genes.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Precise genome engineering can be efficiently made using the revolutionary tool named CRISPR/Cas (clustered regularly interspaced short palindromic repeat/CRISPR-associated protein) systems. Adapted from the bacterial immune system, CRISPR/Cas systems can generate highly specific double-strand breaks (DSBs) at the target site, and desired sequence modifications can be introduced during the DSB repair process, such as nonhomologous end-joining (NHEJ) or homology-directed repair (HDR) pathways. CRISPR/Cas9 is the most widely used genome editing tool for targeted mutagenesis, precise sequence modification, transcriptional reprogramming, epigenome editing, disease treatment, and many more. The ease of use and high specificity make CRISPR/Cas9 a great tool not only for basic researches but also for crop trait improvements, such as higher grain yield, better tolerance to abiotic stresses, enhanced disease resistance, and better nutritional contents. In this protocol, we present a step-by-step guide to the CRISPR/Cas9-mediated targeted mutagenesis in maize Hi II genotype. Detailed procedures will guide through the essential steps including gRNA design, CRISPR/Cas9 vector construction, Agrobacterium-mediated maize immature embryo transformation, and molecular analysis of the transgenic plants to identify desired mutant lines.
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Registered trials
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