ArticleThe EMBO journal2019
Frozen-hydrated chromatin from metaphase chromosomes has an interdigitated multilayer structure.
Article in The EMBO journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed.
- Resolving interface structure and local internal mechanics of mitotic chromosomes.Nature communications · 2025Article
- Nanoscale analysis of human G1 and metaphase chromatin in situ.The EMBO journal · 2025Article
- Cryoelectron tomography reveals the multiplex anatomy of condensed native chromatin and its unfolding by histone citrullination.Molecular cell · 2023Article
- Hypothesis: The opposing pulling forces exerted by spindle microtubules can cause sliding of chromatin layers and facilitate sister chromatid resolution.Frontiers in genetics · 2023Article
- Structural features of nucleosomes in interphase and metaphase chromosomes.Molecular cell · 2021Article
- Differentially accessible, single copy sequences form contiguous domains along metaphase chromosomes that are conserved among multiple tissues.Molecular cytogenetics · 2021Article
- Engineering nucleosomes for generating diverse chromatin assemblies.Nucleic acids research · 2021Article
- Chromosome inner structure investigation by electron tomography and electron diffraction in a transmission electron microscope.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2021Review
- Linker histone defines structure and self-association behaviour of the 177 bp human chromatosome.Scientific reports · 2021Article
- Cryo-nanoscale chromosome imaging-future prospects.Biophysical reviews · 2020Review
- Near-atomic resolution structures of interdigitated nucleosome fibres.Nature communications · 2020Article
- Mitotic chromosome organization: General rules meet species-specific variability.Computational and structural biotechnology journal · 2020Review
- Revealing chromatin organization in metaphase chromosomes.The EMBO journal · 2019Article
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Abstract
Cryo-electron tomography and small-angle X-ray scattering were used to investigate the chromatin folding in metaphase chromosomes. The tomographic 3D reconstructions show that frozen-hydrated chromatin emanated from chromosomes is planar and forms multilayered plates. The layer thickness was measured accounting for the contrast transfer function fringes at the plate edges, yielding a width of ~ 7.5 nm, which is compatible with the dimensions of a monolayer of nucleosomes slightly tilted with respect to the layer surface. Individual nucleosomes are visible decorating distorted plates, but typical plates are very dense and nucleosomes are not identifiable as individual units, indicating that they are tightly packed. Two layers in contact are ~ 13 nm thick, which is thinner than the sum of two independent layers, suggesting that nucleosomes in the layers interdigitate. X-ray scattering of whole chromosomes shows a main scattering peak at ~ 6 nm, which can be correlated with the distance between layers and between interdigitating nucleosomes interacting through their faces. These observations support a model where compact chromosomes are composed of many chromatin layers stacked along the chromosome axis.
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