Evidence map›Paper›PMID 30609859›Full record

ArticleSensors (Basel, Switzerland)2019

3D Printed Modular Immunofiltration Columns for Frequency Mixing-Based Multiplex Magnetic Immunodetection.

Stefan Achtsnicht, Julia Tödter, Julia Niehues, Matthias Telöken, Andreas Offenhäusser, Hans-Joachim Krause, Florian Schröper

Abstract read
In one paragraph

Article in Sensors (Basel, Switzerland), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Stefan AchtsnichtInstitute of Complex Systems Bioelectronics (ICS-8), Forschungszentrum Jülich, 52425 Jülich, Germany. s.achtsnicht@fz-juelich.de.ORCID 0000-0003-0666-912X
Julia TödterFraunhofer Institute for Molecular Biology and Applied Ecology IME, 52074 Aachen, Germany. Julia.Toedter@ime.fraunhofer.de.
Julia NiehuesFraunhofer Institute for Molecular Biology and Applied Ecology IME, 52074 Aachen, Germany. Julia.Niehues@ime.fraunhofer.de.
Matthias TelökenInstitute of Complex Systems Bioelectronics (ICS-8), Forschungszentrum Jülich, 52425 Jülich, Germany. m.teloeken@fz-juelich.de.
Andreas OffenhäusserInstitute of Complex Systems Bioelectronics (ICS-8), Forschungszentrum Jülich, 52425 Jülich, Germany. a.offenhaeusser@fz-juelich.de.ORCID 0000-0001-6143-2702
Hans-Joachim KrauseInstitute of Complex Systems Bioelectronics (ICS-8), Forschungszentrum Jülich, 52425 Jülich, Germany. h.-j.krause@fz-juelich.de.ORCID 0000-0002-7526-9894
Florian SchröperFraunhofer Institute for Molecular Biology and Applied Ecology IME, 52074 Aachen, Germany. Florian.Schroeper@ime.fraunhofer.de.ORCID 0000-0002-2490-3728

Funding

Bundesministerium für Bildung und Forschung 13N13711 and 13N13712
6 · The paper itself

Abstract

For performing point-of-care molecular diagnostics, magnetic immunoassays constitute a promising alternative to established enzyme-linked immunosorbent assays (ELISA) because they are fast, robust and sensitive. Simultaneous detection of multiple biomolecular targets from one body fluid sample is desired. The aim of this work is to show that multiplex magnetic immunodetection based on magnetic frequency mixing by means of modular immunofiltration columns prepared for different targets is feasible. By calculations of the magnetic response signal, the required spacing between the modules was determined. Immunofiltration columns were manufactured by 3D printing and antibody immobilization was performed in a batch approach. It was shown experimentally that two different target molecules in a sample solution could be individually detected in a single assaying step with magnetic measurements of the corresponding immobilization filters. The arrangement order of the filters and of a negative control did not influence the results. Thus, a simple and reliable approach to multi-target magnetic immunodetection was demonstrated.

Indexed as

MagneticsPrinting, Three-DimensionalAntibodies, ImmobilizedBiosensing TechniquesChromatographyComputer SimulationImmunoassayPathology, MolecularSensitivity and SpecificityAntibodies, Immobilized3D printadditive manufacturingfrequency mixing magnetic detectionmagnetic beadmagnetic sandwich immunoasssay

Identifiers

PMID30609859
PMCPMC6338908

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.