ArticleInternational journal of molecular sciences2019
In Vitro Induction of Tendon-Specific Markers in Tendon Cells, Adipose- and Bone Marrow-Derived Stem Cells is Dependent on TGFβ3, BMP-12 and Ascorbic Acid Stimulation.
Article in International journal of molecular sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 54 citations in OpenAlex.
- Tendon healing in the era of regenerative medicine: literature review.Annals of translational medicine · 2026Review
- Insights into vitamin C in musculoskeletal physiology and disorders: mechanisms and translational perspectives.Frontiers in immunology · 2026Review
- Review
- The Application of Photobiomodulation on Mesenchymal Stem Cells and its Potential Use for Tenocyte Differentiation.Current stem cell research & therapy · 2025Review
- Mechanobiological Strategies to Enhance Ovine (Animals : an open access journal from MDPI · 2024Article
- Effect of TGF-β3 on wound healing of bone cell monolayer in static and hydrodynamic shear stress conditions.Frontiers in medicine · 2024Article
- Understanding Fibrous Tissue in the Effective Healing of Rotator Cuff Injury.Journal of surgery and research · 2024Article
- A systematic investigation of the effects of TGF-β3 and mechanical stimulation on tenogenic differentiation of mesenchymal stromal cells in a poly(ethylene glycol)/gelatin-based hydrogel.Journal of orthopaedic translation · 2023Article
- Directing iPSC differentiation into iTenocytes using combined scleraxis overexpression and cyclic loading.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2023Article
- Article
- In Vitro and In Vivo Effects of IGF-1 Delivery Strategies on Tendon Healing: A Review.International journal of molecular sciences · 2023Review
- Growth factors in the treatment of Achilles tendon injury.Frontiers in bioengineering and biotechnology · 2023Review
- Stem Cell Applications and Tenogenic Differentiation Strategies for Tendon Repair.Stem cells international · 2023Review
- Assessing the combined effect of surface topography and substrate rigidity in human bone marrow stem cell cultures.Engineering in life sciences · 2022Article
- The effects of orthobiologics in the treatment of tendon pathologies: a systematic review of preclinical evidence.Journal of experimental orthopaedics · 2022Review
- Development and Regeneration of Muscle, Tendon, and Myotendinous Junctions in Striated Skeletal Muscle.International journal of molecular sciences · 2022Review
- State of Knowledge on Molecular Adaptations to Exercise in Humans: Historical Perspectives and Future Directions.Comprehensive Physiology · 2022Article
- Advanced Nanofiber-Based Scaffolds for Achilles Tendon Regenerative Engineering.Frontiers in bioengineering and biotechnology · 2022Review
- Advances in Stem Cell Therapies for Rotator Cuff Injuries.Frontiers in bioengineering and biotechnology · 2022Review
- Growth factor and macromolecular crowding supplementation in human tenocyte culture.Biomaterials and biosystems · 2021Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mesenchymal Stem Cells (MSCs) and tissue-specific progenitors have been proposed as useful tools for regenerative medicine approaches in bone, cartilage and tendon-related pathologies. The differentiation of cells towards the desired, target tissue-specific lineage has demonstrated advantages in the application of cell therapies and tissue engineering. Unlike osteogenic and chondrogenic differentiation, there is no consensus on the best tenogenic induction protocol. Many growth factors have been proposed for this purpose, including BMP-12, b-FGF, TGF-β3, CTGF, IGF-1 and ascorbic acid (AA). In this study, different combinations of these growth factors have been tested in the context of a two-step differentiation protocol, in order to define their contribution to the induction and maintenance of tendon marker expression in adipose tissue and bone marrow derived MSCs and tendon cells (TCs), respectively. Our results demonstrate that TGF-β3 is the main inducer of scleraxis, an early expressed tendon marker, while at the same time inhibiting tendon markers normally expressed later, such as decorin. In contrast, we find that decorin is induced by BMP-12, b-FGF and AA. Our results provide new insights into the effect of different factors on the tenogenic induction of MSCs and TCs, highlighting the importance of differential timing in TGF-β3 stimulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.