ArticleBiomacromolecules2019
Synthetic Extracellular Matrices with Nonlinear Elasticity Regulate Cellular Organization.
Article in Biomacromolecules, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers, 2 of them syntheses that pooled it.
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Who cites it
43 citing papers in PubMed, 2 syntheses or guidelines pooled it, 93 citations in OpenAlex.
- Systematic review on spheroids from adipose-derived stem cells: Spontaneous or artefact state?Journal of cellular physiology · 2022Pooled it
- Pooled it
- In situ processing of fibrous hydrogels by photochemistry for adaptive cellular microenvironments.Science advances · 2026Article
- A Water-Soluble Fluorogenic Probe to Quantify Azide Concentrations in Biologically Relevant Environments.Biomacromolecules · 2026Article
- Decoupling Dynamics and Crosslink Stability in Supramolecular Hydrogels Using Associative Exchange.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Next-Generation Hydrogels for Biliary Organoid Engineering.Pharmaceuticals (Basel, Switzerland) · 2025Review
- Fibrous polyisocyanide hydrogels for 3D cell culture applications.Nature protocols · 2025Review
- Invasin-functionalized PIC hydrogels enable long-term 3D culture of epithelial organoids.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- The role of non-linear viscoelastic hydrogel mechanics in cell culture and transduction.Materials today. Bio · 2025Review
- Revisiting the biophysical aspects of extracellular-matrix-mimicking hydrogels: what cells seeBiomaterials science · 2025Review
- Tunable hydrogel networks by varying secondary structures of hydrophilic peptoids provide viable 3D cell culture platforms for hMSCs.Biomaterials science · 2025Article
- Nonlinear Elastic Bottlebrush Polymer Hydrogels Modulate Actomyosin Mediated Protrusion Formation in Mesenchymal Stromal Cells.Advanced materials (Deerfield Beach, Fla.) · 2024Article
- Tailoring of Physical Properties in Macroporous Poly(isocyanopeptide) Cryogels.Biomacromolecules · 2024Article
- Article
- An Improved Understanding of the Pathophysiology of Pelvic Organ Prolapse: A 3D In Vitro Model under Static and Mechanical Loading Conditions.Advanced healthcare materials · 2024Article
- Automated Nanodroplet Dispensing for Large-Scale Spheroid Generation via Hanging Drop and Parallelized Lossless Spheroid Harvesting.Micromachines · 2024Article
- Exploring the interaction between extracellular matrix components in a 3D organoid disease model to replicate the pathophysiology of breast cancer.Journal of experimental & clinical cancer research : CR · 2023Review
- Growth Factor Immobilization to Synthetic Hydrogels: Bioactive bFGF-Functionalized Polyisocyanide Hydrogels.Advanced healthcare materials · 2023Article
- Vaginal Fibroblast Behavior as a Function of Stiffness Changes in a Polyisocyanide Hydrogel for Prolapse Repair.ACS applied bio materials · 2023Article
- Recent Advances in Tissue-Engineered Cardiac Scaffolds-The Progress and Gap in Mimicking Native Myocardium Mechanical Behaviors.Journal of functional biomaterials · 2023Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One of the promises of synthetic materials in cell culturing is that control over their molecular structures may ultimately be used to control their biological processes. Synthetic polymer hydrogels from polyisocyanides (PIC) are a new class of minimal synthetic biomaterials for three-dimensional cell culturing. The macromolecular lengths and densities of biofunctional groups that decorate the polymer can be readily manipulated while preserving the intrinsic nonlinear mechanics, a feature commonly displayed by fibrous biological networks. In this work, we propose the use of PIC gels as cell culture platforms with decoupled mechanical inputs and biological cues. For this purpose, different types of cells were encapsulated in PIC gels of tailored compositions that systematically vary in adhesive peptide (GRGDS) density, polymer length, and concentration; with the last two parameters controlling the gel mechanics. Both cancer and smooth muscle cells grew into multicellular spheroids with proliferation rates that depend on the adhesive GRGDS density, regardless of the polymer length, suggesting that for these cells, the biological input prevails over the mechanical cues. In contrast, human adipose-derived stem cells do not form spheroids but rather spread out. We find that the morphological changes strongly depend on the adhesive ligand density and the network mechanics; gels with the highest GRGDS densities and the strongest stiffening response to stress show the strongest spreading. Our results highlight the role of the nonlinear mechanics of the extracellular matrix and its synthetic mimics in the regulation of cell functions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.