ArticleParasitology research2019
Real-time PCR detection of Toxoplasma gondii in surface water samples in São Paulo, Brazil.
Article in Parasitology research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Evolution ofJournal of parasitology research · 2026Review
- Leveraging CRISPR-Cas-Enhanced Isothermal Amplification Tools for Quick Identification of Pathogens Causing Livestock Diseases.Current microbiology · 2025Review
- First report of molecular detection and phylogenetic analysis of Toxoplasma Gondii in soil, water and vegetables from Chandigarh city, India.Scientific reports · 2025Article
- A novel single-tube LAMP-CRISPR/Cas12b method for rapid and visual detection of zoonotic Toxoplasma gondii in the environment.Infectious diseases of poverty · 2024Article
- Trend in serological and molecular diagnostic methods for Toxoplasma gondii infection.European journal of medical research · 2024Review
- Associations between Fetal Symptoms during Pregnancy and Neonatal Clinical Complications with Toxoplasmosis.Children (Basel, Switzerland) · 2024Article
- Review
- Article
- Analytical sensitivity of a multiplex quantitative PCR for Toxoplasma gondii and Neospora caninum.Parasitology research · 2023Article
- Molecular Identification of ProtozoanLife (Basel, Switzerland) · 2022Article
- Detection of DNA from Toxoplasma gondii oocysts in water for reuse.Parasitology research · 2022Article
- Investigation of Toxoplasma gondii in wastewater and surface water in the Qinghai-Tibet Plateau, China using real-time PCR and multilocus genotyping.Scientific reports · 2022Article
- Contamination of Soil, Water, Fresh Produce, and Bivalve Mollusks withMicroorganisms · 2022Review
- Detection of Toxoplasma gondii in water for human consumption from water wells (jagüeyes) in the rural area of the municipality of SincelejoBiomedica : revista del Instituto Nacional de Salud · 2021Article
- Molecular Methods for the Detection ofMicroorganisms · 2021Review
- Determination of the viability of Toxoplasma gondii oocysts by PCR real-time after treatment with propidium monoazide.Revista do Instituto de Medicina Tropical de Sao Paulo · 2020Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Water is considered an important vehicle for the spread of human toxoplasmosis in several countries. Toxoplasma gondii oocysts can persist in the environment for long periods, being highly resistant to the various chemical inactivation processes commonly used by water supply systems, distinctly from simple filtration and flocculation that are efficient in removing oocysts from drinking water. The existing methodologies for identification and quantification of this parasite in water samples are not standardized and have limitations. This study aimed to evaluate the presence of T. gondii oocysts in surface water samples used as a source for the production of drinking water in the State of São Paulo, through the implementation of a specific methodology using real-time PCR technique (qPCR). Volumes of 20 L of the sample were concentrated by filtration in Envirocheck® HV capsules. For DNA extraction, the PowerSoil DNA isolation® kit (currently DNeasy PowerSoil®) was used. The target sequence selected for qPCR was a 62-base-pair fragment of the B1 gene. In the initial recovery evaluation of the method in four replicates of reverse osmosis water, the mean recovery was 48.5% (SD ± 11.5), while the mean recovery for method performance in matrices was 3.2% (SD ± 3.2) (rainy season) and 62.0% (SD ± 6.2) (dry period), suggesting that the characteristics of the samples and the climatic conditions interfere in the recovery efficiency. Of the 39 samples analyzed (May to December 2015), 7.7% (3/39) were positive for T. gondii, and among the ten sources studied; the occurrence of the oocysts was detected in 30% (3/10).
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