Evidence map›Paper›PMID 30607178›Full record

ArticleOpen access Macedonian journal of medical sciences2018

Isolation and Genotyping of Acanthamoeba from Soil Samples in Markazi Province, Iran.

Mehri Meighani, Zahra Eslamirad, Reza Hajihossein, Azam Ahmadi, Sassan Saki

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Article in Open access Macedonian journal of medical sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Genotyping ofIranian journal of parasitology
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Mehri MeighaniDepartment of Biology, School of Basic Sciences, Islamic Azad University, Arak Branch, Arak, Iran.
Zahra EslamiradMolecular and Medicine Research Center, Department of Medical Parasitology and Mycology, School of Medicine, Arak University of Medical Sciences, Arak, Iran.
Reza HajihosseinDepartment of Medical Parasitology and Mycology, School of Medicine, Arak University of Medical Sciences, Arak, Iran.
Azam AhmadiInfectious Disease Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran.
Sassan SakiDepartment of Medical Laboratory Sciences, Faculty of Medical Sciences, Islamic Azad University, Arak Branch, Arak, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimA previous study confirmed the contamination of water sources with this parasite in Arak, Markazi Province, Iran. The current study investigated soil sources and determined the predominant genotype of Acanthamoeba in this region of Iran. MATERIAL AND

methodsForty-eight soil samples, collected from different regions of Arak, Markazi province, Iran, were evaluated in this study. The samples were processed and identified by culturing on a specific medium, performing PCR assay, and sequencing the PCR products. Finally, using the NCBI database, the genotypes were determined.

resultsOf 48 soil samples, 33.3% and 31.25% were contaminated with Acanthamoeba according to the culture and molecular assays, respectively. The majority of these isolates belonged to the T4, T5 and T6 genotypes of Acanthamoeba.

conclusionThe genotypes of most isolates from soil samples in Arak similar to other regions of Iran belong to T4 genotype of this parasite. New sequence accession numbers include MG066681 and MG298785-MG298794.

Indexed as

AcanthamoebaGenotypeMolecular methodPCRSoil

Identifiers

PMID30607178
PMCPMC6311493

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