ArticleReproductive biology and endocrinology : RB&E2019
Profile of melatonin and its receptors and synthesizing enzymes in cumulus-oocyte complexes of the developing sheep antral follicle-a potential estradiol-mediated mechanism.
Article in Reproductive biology and endocrinology : RB&E, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 94 citations in OpenAlex.
- Review
- Follicular dynamics, cumulus-oocyte complex recovery, and reproductive resilience in local Algerian ewes during seasonal anestrus.Tropical animal health and production · 2026Article
- The Critical Functions of FGF2, LIF and IGF1 in the Improvement of In Vitro Embryo Production.Biomolecules · 2026Review
- Decoding the Role of Kinesin Superfamily Proteins in Glioma Progression.Journal of molecular neuroscience : MN · 2025Review
- Variation in theAnimal biotechnology · 2024Article
- Modulation of Melatonin Receptors Regulates Reproductive Physiology: The Impact of Agomelatine on the Estrus Cycle, Gestation, Offspring, and Uterine Contractions in Rats.Physiological research · 2023Article
- The Effect on Inflammation of Adherence to the Mediterranean Diet in Polycystic Ovary Syndrome.Current nutrition reports · 2023Review
- Dietary flaxseed oil and vitamin E improve semen qualityFrontiers in endocrinology · 2023Article
- Deregulated Brain's Central Clock Management on Sleep-Wake Behavior in Women With Polycystic Ovary Syndrome: Melatonin & Sleep Pattern.Journal of family & reproductive health · 2022Review
- Melatonin Receptors: A Key Mediator in Animal Reproduction.Veterinary sciences · 2022Review
- Exogenous Melatonin Directly and Indirectly Influences Sheep Oocytes.Frontiers in veterinary science · 2022Article
- Ovarian activation delays in peripubertal ewe lambs infected with Haemonchus contortus can be avoided by supplementing protein in their diets.BMC veterinary research · 2021Article
- Research progress of melatonin (MT) in improving ovarian function: a review of the current status.Aging · 2021Article
- Melatonin Represses Mitophagy to Protect Mouse Granulosa Cells from Oxidative Damage.Biomolecules · 2021Article
- Meta-analysis of melatonin treatment and porcine somatic cell nuclear transfer embryo development.Animal reproduction · 2021Review
- Article
- Role of Melatonin in the Regulation of Pain.Journal of pain research · 2020Review
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9 authors at 1 institution in 1 country.
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Abstract
backgroundMelatonin is an amine hormone that plays an important role in regulating mammalian reproduction. This study aimed to investigate the expression pattern of melatonin synthesis enzymes AANAT and HIOMT and melatonin receptors MT1 and MT2 in sheep cumulus-oocyte complexes (COCs) as well as the change of melatonin level in follicular fluid (FF) during antral follicle development. In this research, we also study the effect of β-estradiol (E2) on MT1 and MT2 expression as well as melatonin synthesis in COCs so as to lay the foundation for further exploration of the regulation mechanism of melatonin synthesis in the ovary.
methodsCOCs and FF were collected from different size (large follicles (diameter ≥ 5 mm), medium follicles (diameter 2-5 mm), and small follicles (diameter ≤ 2 mm)) of antral follicles in sheep ovaries. To assess whether E2 regulates melatonin synthase and its receptors expression in sheep COCs and whether it is mediated through estrogen receptor (ER) pathway. The collected COCs were cultured in vitro for 24 h and then treat with 1 μM E2 and/or 1 μM ICI182780 (non-selective ER antagonist). The expression of AANAT, HIOMT, MT1 and MT2 mRNA and protein were determined by qRT-PCR and western blot. The melatonin level was determined by ELISA.
resultsThe expression of AANAT, HIOMT, MT1 and MT2 were significantly higher expression in the COCs of small follicles than in those of large follicles (P < 0.05). However, the melatonin level was significantly higher in large follicle FF than in small follicle FF (P < 0.05). Further, the expression of AANAT, HIOMT, MT1, and MT2 and melatonin production were decreased by E2 treatment (P < 0.05), but when ICI182780 was added, the expression of AANAT, HIOMT, MT1, and MT2 and melatonin production recovered (P < 0.05).
conclusionsWe suggest that sheep COCs can synthesize melatonin, but this ability is decreased with increasing follicle diameter. Furthermore, E2 play an important role in regulated the expression of MT1 and MT2 as well as melatonin synthesis in sheep COCs through the ER pathway.
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