ArticleCell reports2019
Flexible Nanopipettes for Minimally Invasive Intracellular Electrophysiology In Vivo.
Article in Cell reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 63 citations in OpenAlex.
- Neuronal ensembles in cortical function and disease.Physiological reviews · 2026Review
- Intracellular neuronal recordings across DNA tiles.Nature nanotechnology · 2026Article
- Electrophysiological classification of human layer 2-3 pyramidal neurons reveals subtype-specific synaptic interactions.Nature neuroscience · 2026Article
- Nanopore-Based Neurotransmitter Detection: Advances, Challenges, and Future Perspectives.ACS nano · 2025Review
- Materials and device strategies to enhance spatiotemporal resolution in bioelectronics.Nature reviews. Materials · 2025Article
- Site-specific seeding of Lewy pathology induces distinct pre-motor cellular and dendritic vulnerabilities in the cortex.Nature communications · 2024Article
- Parvalbumin interneuron impairment causes synaptic transmission deficits and seizures in SCN8A developmental and epileptic encephalopathy.JCI insight · 2024Article
- Probing multiplexed basal dendritic computations using two-photon 3D holographic uncaging.Cell reports · 2024Article
- Membrane-coated glass electrodes for stable, low-noise electrophysiology recordings in Drosophila central neurons.Journal of neuroscience methods · 2024Article
- Intracellular neural control of an active feeding structure in Aplysia using a carbon fiber electrode array.Journal of neuroscience methods · 2024Article
- Epilepsy insights revealed by intravital functional optical imaging.Frontiers in neurology · 2024Review
- Diversity of dynamic voltage patterns in neuronal dendrites revealed by nanopipette electrophysiology.Nanoscale · 2023Article
- Integration of reconfigurable microchannels into aligned three-dimensional neural networks for spatially controllable neuromodulation.Science advances · 2023Article
- Seizures initiate in zones of relative hyperexcitation in a zebrafish epilepsy model.Brain : a journal of neurology · 2022Article
- Simultaneous two-photon imaging of action potentials and subthreshold inputs in vivo.Nature communications · 2021Article
- Learning from the brain's architecture: bioinspired strategies towards implantable neural interfaces.Current opinion in biotechnology · 2021Review
- Somatostatin-Positive Interneurons Contribute to Seizures inThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Time for NanoNeuro.Nature methods · 2021Review
- Modeling the voltage distribution in a non-locally but globally electroneutral confined electrolyte medium: applications for nanophysiology.Journal of mathematical biology · 2021Article
- Measuring Absolute Membrane Potential Across Space and Time.Annual review of biophysics · 2021Review
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
Intracellular recordings in vivo remains the best technique to link single-neuron electrical properties to network function. Yet existing methods are limited in accuracy, throughput, and duration, primarily via washout, membrane damage, and movement-induced failure. Here, we introduce flexible quartz nanopipettes (inner diameters of 10-25 nm and spring constant of ∼0.08 N/m) as nanoscale analogs of traditional glass microelectrodes. Nanopipettes enable stable intracellular recordings (seal resistances of 500 to ∼800 MΩ, 5 to ∼10 cells/nanopipette, and duration of ∼1 hr) in anaesthetized and awake head-restrained mice, exhibit minimal diffusional flux, and facilitate precise recording and stimulation. When combined with quantum-dot labels and microprisms, nanopipettes enable two-photon targeted electrophysiology from both somata and dendrites, and even paired recordings from neighboring neurons, while permitting simultaneous population imaging across cortical layers. We demonstrate the versatility of this method by recording from parvalbumin-positive (Pv) interneurons while imaging seizure propagation, and we find that Pv depolarization block coincides with epileptic spread. Flexible nanopipettes present a simple method to procure stable intracellular recordings in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.