Evidence map›Paper›PMID 3060468›Full record

ArticleThe Journal of cell biology1988

Yeast actin-binding proteins: evidence for a role in morphogenesis.

D G Drubin, K G Miller, D Botstein

Open access · bronzeAbstract read
In one paragraph

Article in The Journal of cell biology, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 151 papers.

0numbers the graph read from it
0cells of the map it votes in
151citing papers in PubMed
8.3field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

151 citing papers in PubMed, 305 citations in OpenAlex.

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  8. Profile of David G. Drubin.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
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  10. ER-phagy requires the assembly of actin at sites of contact between the cortical ER and endocytic pits.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
  11. Article
  12. Genetically inspired in vitro reconstitution ofMolecular biology of the cell · 2020
    Article
  13. Article
  14. Copper Resistance inFrontiers in microbiology · 2017
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91 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

D G DrubinDepartment of Biology, Massachusetts Institute of Technology, Cambridge 02139.
K G Miller
D Botstein
Massachusetts Institute of Technology · US

Funding

MOLECULAR GENETICS OF MORPHOGENETIC PATHWAYSR01GM021253 · NIGMS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOTSTEIN, DAVID · 1985 to 1989
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NIGMS NIH HHS GM08740NIGMS NIH HHS GM18973NIGMS NIH HHS GM21253
6 · The paper itself

Abstract

Three yeast actin-binding proteins were identified using yeast actin filaments as an affinity matrix. One protein appears to be a yeast myosin heavy chain; it is dissociated from actin filaments by ATP, it is similar in size (200 kD) to other myosins, and antibodies directed against Dictyostelium myosin heavy chain bind to it. Immunofluorescence experiments show that a second actin-binding protein (67 kD) colocalizes in vivo with both cytoplasmic actin cables and cortical actin patches, the only identifiable actin structures in yeast. The cortical actin patches are concentrated at growing surfaces of the yeast cell where they might play a role in membrane and cell wall insertion, and the third actin-binding protein (85 kD) is only detected in association with these structures. This 85-kD protein is therefore a candidate for a determinant of growth sites. The in vivo role of this protein was tested by overproduction; this overproduction causes a reorganization of the actin cytoskeleton which in turn dramatically affects the budding pattern and spatial growth organization of the yeast cell.

Indexed as

ActinsAutoradiographyElectrophoresis, Polyacrylamide GelFluorescent Antibody TechniqueImmunoblottingMicrofilament ProteinsMorphogenesisMyosinsSaccharomyces cerevisiaeActinsMicrofilament ProteinsMyosins

Identifiers

PMID3060468
PMCPMC2115664
OpenAlexW2164021052

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.