Evidence map›Paper›PMID 30604276›Full record

ArticleWorld journal of microbiology & biotechnology2019

Bioprospecting of whole-cell biocatalysts for cholesterol biotransformation.

Victoria Giorgi, Michel Chaves, Pilar Menéndez, Carlos García Carnelli

Abstract read
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In one paragraph

Article in World journal of microbiology & biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Victoria GiorgiLaboratorio de Biocatálisis y Biotransformaciones, Departamento de Química Orgánica y, Departamento de Biociencias, Facultad de Química, Universidad de la República (UdelaR), CP 11800, Montevideo, Uruguay. vgiorgi@fq.edu.uy.ORCID http://orcid.org/0000-0002-9408-3585
Michel ChavesLaBioChem, Institute of Chemistry, University of Campinas, Campinas, SP, 13084-971, Brazil.
Pilar MenéndezLaboratorio de Biocatálisis y Biotransformaciones, Departamento de Química Orgánica y, Departamento de Biociencias, Facultad de Química, Universidad de la República (UdelaR), CP 11800, Montevideo, Uruguay.
Carlos García CarnelliLaboratorio de Biocatálisis y Biotransformaciones, Departamento de Química Orgánica y, Departamento de Biociencias, Facultad de Química, Universidad de la República (UdelaR), CP 11800, Montevideo, Uruguay.

Funding

Agencia Nacional de Investigación e Innovación POS_NAC_2013_1_11432Comisión Académica de Posgrado(UdelaR) -Comisión Sectorial de Investigación Científica -Programa de Desarrollo de las Ciencias Básicas (PEDECIBA) _Sociedad Uruguaya de Biociencias (UY) -
6 · The paper itself

Abstract

Microorganisms were isolated from industrial wool scouring effluents and from the soil adjacent to the wastewater treatment lagoon, both sterols-rich environments, in order to search for novel biocatalysts able to transform cholesterol. The isolates were identified on the basis of morphological and biochemical characteristics and phylogenetic analysis. Furthermore, a rapid and accurate bacteria identification by matrix assisted laser desorption/ionization-time-of-flight mass spectrometry was carried out. Bacteria and fungi including representatives of the genera Fusarium, Talaromyces, Trichoderma, Mucor, Aspergillus, Citrobacter, Proteus, Klebsiella, Exiguobacterium, Acinetobacter, Tsukamurella, Bacillus, and Streptomyces were found and evaluated for their ability to biotransform cholesterol by whole-cell treatment system. The results show that a Trichoderma koningiopsis strain, as well as two strains of Mucor circinelloides were able to transform cholesterol into value-added products. The major products were characterized as 7β-hydroxycholesterol, 4-cholesten-3-one, 5α,6α-epoxycholestan-3β-ol and 5β,6β-epoxycholestan-3β-ol. To the best of our knowledge, the present study is the first report of cholesterol biotransformation by representatives of Trichoderma and Mucor genera.

Indexed as

BioprospectingBiotransformationCatalysisCholesterolSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationTrichodermaCholesterolBiotransformationCholesterolMALDI-TOF MSMucorTrichodermaWhole-cell biocatalyst

Identifiers

PMID30604276

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.