Evidence map›Paper›PMID 30603591›Full record

ArticleTissue engineering and regenerative medicine2018

Keratinocyte Migration in a Three-Dimensional

Kritika Iyer, Zhuo Chen, Teja Ganapa, Benjamin M Wu, Bill Tawil, Chase S Linsley

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Tissue engineering and regenerative medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
2.6field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 37 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Kritika Iyer1Department of Bioengineering, University of California, Los Angeles, 420 Westwood Plaza, Room 5121, Engineering V, P.O. Box 951600, Los Angeles, CA 90095-1600 USA.
Zhuo Chen1Department of Bioengineering, University of California, Los Angeles, 420 Westwood Plaza, Room 5121, Engineering V, P.O. Box 951600, Los Angeles, CA 90095-1600 USA.
Teja Ganapa1Department of Bioengineering, University of California, Los Angeles, 420 Westwood Plaza, Room 5121, Engineering V, P.O. Box 951600, Los Angeles, CA 90095-1600 USA.
Benjamin M Wu1Department of Bioengineering, University of California, Los Angeles, 420 Westwood Plaza, Room 5121, Engineering V, P.O. Box 951600, Los Angeles, CA 90095-1600 USA.
Bill Tawil1Department of Bioengineering, University of California, Los Angeles, 420 Westwood Plaza, Room 5121, Engineering V, P.O. Box 951600, Los Angeles, CA 90095-1600 USA.
Chase S Linsley1Department of Bioengineering, University of California, Los Angeles, 420 Westwood Plaza, Room 5121, Engineering V, P.O. Box 951600, Los Angeles, CA 90095-1600 USA.ORCID 0000-0002-8259-5487
University of California, Los Angeles · US

Funding

Growth factor delivery using light-sensitive fibrin to treat chronic woundsR21AR064437 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI TAWIL, BILL, WU, BENJAMIN M · 2014 to 2015
$359k
NIAMS NIH HHS R21 AR064437
6 · The paper itself

Abstract

backgroundBecause three-dimensional (3D) models more closely mimic native tissues, one of the goals of 3D

methodsOptical imaging was used to measure keratinocyte migration in the presence of fibroblasts over 7 days onto the fibrin-filled defects. Additionally, cell viability and growth of fibroblasts and keratinocytes was measured using the alamarBlue

resultsKeratinocyte migration rate was significantly increased in the presence of fibroblasts with the cells reaching the center of the defect as early as day 3 in the co-culture constructs compared to day 7 for the control keratinocyte monoculture constructs. Additionally, constructs with the greatest rate of keratinocyte migration had reduced cell growth. When fibroblasts were cultured alone in the wound healing construct, there was a 1.3 to 3.4-fold increase in cell growth and a 1.2 to 1.4-fold increase in cell growth for keratinocyte monocultures. However, co-culture constructs exhibited no significant growth over 7 days. Finally, mechanical testing showed that fibroblasts and keratinocytes had varying effects on matrix stiffness with fibroblasts degrading the constructs while keratinocytes increased the construct's stiffness.

conclusionThis 3D

Indexed as

Cell migrationCell proliferationCollagenFibrinIn vitro model

Identifiers

PMID30603591
PMCPMC6250652
OpenAlexW2887131988

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.