ArticleTissue engineering and regenerative medicine2018
Optimal Stem Cell Transporting Conditions to Maintain Cell Viability and Characteristics.
Article in Tissue engineering and regenerative medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Comprehensive evaluation of the clinical feasibility of using perinatal medical waste as a source for fetal mesenchymal stem cell banking under good manufacturing practice conditions.Cell and tissue banking · 2025Observational
- Quality assessment strategy development and analytical method selection of GMP grade biological drugs for gene and cell therapy.BBA advances · 2025Review
- Optimizing transport methods to preserve function of self-innervating muscle cells for laryngeal injection.Laryngoscope investigative otolaryngology · 2024Article
- Understanding and controlling the variables for stromal vascular fraction therapy.World journal of stem cells · 2024Review
- Article
- Fabrication of three-lobed magnetic microrobots for cell transportation.Journal of materials chemistry. B · 2023Article
- Within or Without You? A Perspective Comparing In Situ and Ex Situ Tissue Engineering Strategies for Articular Cartilage Repair.Advanced healthcare materials · 2022Article
- Challenges of Cellular Therapy During the COVID-19 Pandemic.Advances in experimental medicine and biology · 2021Article
- Article
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe preservation of stem cell viability and characteristics during cell transport from the bench to patients can significantly affect the success of cell therapy. Factors such as suspending medium, time, temperature, cell density, and container type could be considered for transport conditions.
methodsTo establish optimal conditions, human amniotic fluid stem cells' (AFSCs) viabilities were analyzed under different media {DMEM(H), DMEM/F-12, K-SFM, RPMI 1640, α-MEM, DMEM(L), PBS or saline}, temperature (4, 22 or 37 °C), cell density (1 × 10
resultsCells transported in DMEM(H) showed relatively higher viability than other media. The optimized transport temperature was 4 °C, and available transport time was within 12 h. A lower cell density was associated with a better survival rate, and a syringe was selected as a transport container because of its clinical convenience. In compare of stem cell characteristics, the transported cells with established conditions showed similar potency as the freshly prepared cells.
conclusionOur findings can provide a foundation to optimization of conditions for stem cell transport.
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Registered trials
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