Evidence map›Paper›PMID 30602968›Full record

ArticleAfrican health sciences2018

Problems encountered in conventional HIV 1/2 Algorithms: lack of necessity for immunoblot assays to confirm repeated ELISA reactive results.

Pelin Yuksel, Suat Saribas, Mert Kuskucu, Sibel Islak Mutcali, Erdogan Kosan, Zafer Habip, Mehmet Demirci, Eda Salihoglu Kara, Ilhan Birinci, Reyhan Caliskan and 4 more

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Article in African health sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Pelin YukselIstanbul University, Cerrahpaşa Medical Faculty, Department of Medical Microbiology, Istanbul/Turkey.
Suat SaribasIstanbul University, Cerrahpaşa Medical Faculty, Department of Medical Microbiology, Istanbul/Turkey.
Mert KuskucuIstanbul University, Cerrahpaşa Medical Faculty, Department of Medical Microbiology, Istanbul/Turkey.
Sibel Islak MutcaliIstanbul Venerial Disease and Leprosy Hospital, Istanbul, Turkey.
Erdogan KosanThe Turkish Red Crescent Marmara Region Blood Center Laboratory, Istanbul/ Turkey.
Zafer HabipIstanbul University, Cerrahpaşa Medical Faculty, Department of Medical Microbiology, Istanbul/Turkey.
Mehmet DemirciBeykent University Medical Faculty, Department of Medical Microbiology, Istanbul, Turkey.
Eda Salihoglu KaraBakırköy Mazhar Osman Research and Training Hospital for Psychiatry, Neurology and Neurosurgery, Istanbul, Turkey.
Ilhan BirinciThe Turkish Red Crescent Marmara Region Blood Center Laboratory, Istanbul/ Turkey.
Reyhan CaliskanIstanbul University, Cerrahpaşa Medical Faculty, Department of Medical Microbiology, Istanbul/Turkey.
Harika Oyku DincIstanbul University, Cerrahpaşa Medical Faculty, Department of Medical Microbiology, Istanbul/Turkey.
Kenan MidilliIstanbul University, Cerrahpaşa Medical Faculty, Department of Medical Microbiology, Istanbul/Turkey.
Tevhide ZiverEast Mediterranean University, Health Sciences Faculty, Gazimagusa, North Cyprus.
Bekir KocazeybekIstanbul University, Cerrahpaşa Medical Faculty, Department of Medical Microbiology, Istanbul/Turkey.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe use of conventional (serologically based) HIV 1/2 diagnostic algorithms has become controversial in recent years.

objectivesSera from patients who underwent verification tests were evaluated because repeated ELISA-reactive results demonstrated a HIV1+HIV2 positive band pattern.

methodsThe line immunoassay (LIA) test was used for repeated HIV enzyme immunoassays (EIA)-reactive sera in patients at three centers. The Bio-Rad Geenius™ HIV 1/2 and the HIV-1 RNA tests were used. HIV-1 and RNA HIV-2 were investigated using PCR.

resultsLIA was used to evaluate 3,224 out of 10,591 samples with repeated ELISA reactivity (30%). We found that 32 (1%) of the sera, along with HIV1 bands and HIV2 gp36 bands, were positive. Only 28 of the 32 verified serum samples with gp36 bands were repeated, and no gp36 band positivity was detected using the Bio-Rad Geenius™ HIV-1/2 confirmatory assay in these serum samples. The HIV-2 proviral DNAs were also negative. Therefore, we excluded the possibility of HIV1+2 co-infection. All samples from the 32 patients were positive for HIV-1 RNA.

conclusionOur findings highlight the need to exclude confirmatory tests like the LIA test from the current diagnostic HIV algorithm and replace it with rapid HIV-1 and HIV-2 confirmatory immunochromotographic tests.

Indexed as

AdolescentAdultAIDS SerodiagnosisBlotting, WesternCross-Sectional StudiesEnzyme-Linked Immunosorbent AssayFemaleHIV-1HIV-2HIV InfectionsHumansImmunoassayImmunoblottingMaleMiddle AgedSensitivity and SpecificityAIDSHIVHIV-2

Identifiers

PMID30602968
PMCPMC6306965

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.