ArticlePlant molecular biology2019
Analysis of transcripts and splice isoforms in Medicago sativa L. by single-molecule long-read sequencing.
Article in Plant molecular biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 59 citations in OpenAlex.
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- Biological Insights and Recent Advances in Plant Long Non-Coding RNA.International journal of molecular sciences · 2024Review
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- Full-Length Transcriptome Sequencing Reveals the Molecular Mechanism ofAntioxidants (Basel, Switzerland) · 2023Article
- Screening and identification of multiple abiotic stress responsive candidate genes based on hybrid-sequencing inHeliyon · 2023Article
- Microsatellite analysis and polymorphic marker development based on the full-length transcriptome of Camellia chekiangoleosa.Scientific reports · 2022Article
- Combination analysis of single-molecule long-read and Illumina sequencing provides insights into the anthocyanin accumulation mechanism in an ornamental grass, Pennisetum setaceum cv. Rubrum.Plant molecular biology · 2022Article
- Comparative Transcriptome Analysis Reveals New Insight of Alfalfa (Frontiers in plant science · 2022Article
- Full-Length Transcriptomics Reveals Complex Molecular Mechanism of Salt Tolerance inFrontiers in plant science · 2022Article
- EST-SSR Marker Development and Full-Length Transcriptome Sequence Analysis of Tiger Lily (Applied bionics and biomechanics · 2022Article
- Genome-Wide Analysis of Long Non-coding RNAs Involved in Nodule Senescence inFrontiers in plant science · 2022Article
- Full-length transcriptome analysis and identification of transcript structures in Eimeria necatrix from different developmental stages by single-molecule real-time sequencing.Parasites & vectors · 2021Article
- Full-length SMRT transcriptome sequencing and microsatellite characterization in Paulownia catalpifolia.Scientific reports · 2021Article
- A Tissue-Specific Landscape of Alternative Polyadenylation, lncRNAs, TFs, and Gene Co-expression Networks inFrontiers in plant science · 2021Article
- The Landscapes of Full-Length Transcripts and Splice Isoforms as Well as Transposons Exonization in the Lepidopteran Model System,Frontiers in genetics · 2021Article
- Revealing the full-length transcriptome of caucasian clover rhizome development.BMC plant biology · 2020Article
- Single-molecule long-read sequencing of the full-length transcriptome of Rhododendron lapponicum L.Scientific reports · 2020Article
- PacBio single-molecule long-read sequencing shed new light on the transcripts and splice isoforms of the perennial ryegrass.Molecular genetics and genomics : MGG · 2020Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
key messageThe full-length transcriptome of alfalfa was analyzed with PacBio single-molecule long-read sequencing technology. The transcriptome data provided full-length sequences and gene isoforms of transcripts in alfalfa, which will improve genome annotation and enhance our understanding of the gene structure of alfalfa. As an important forage, alfalfa (Medicago sativa L.) is world-wide planted. For its complexity of genome and unfinished whole genome sequencing, the sequences and complete structure of mRNA transcripts remain unclear in alfalfa. In this study, single-molecule long-read sequencing was applied to investigate the alfalfa transcriptome using the Pacific Biosciences platform, and a total of 113,321 transcripts were obtained from young, mature and senescent leaves. We identified 72,606 open reading frames including 46,616 full-length ORFs, 1670 transcription factors from 54 TF families and 44,040 simple sequence repeats from 30,797 sequences. A total of 7568 alternative splicing events was identified and the majority of alternative splicing events in alfalfa was intron retention. In addition, we identified 17,740 long non-coding RNAs. Our results show the feasibility of deep sequencing full-length RNA from alfalfa transcriptome on a single-molecule level.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.