ArticleThe Review of scientific instruments2018
Theoretical analysis of spectral precision in spectroscopic single-molecule localization microscopy.
Article in The Review of scientific instruments, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 23 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Solvatochromic BODIPYs with Polarity-Independent Absorption for Functional Super-resolution Imaging of Live Cell Membranes.Chemical & biomedical imaging · 2026Article
- Spectroscopic DNA-PAINT for simultaneous multiplexed super-resolution microscopy.bioRxiv : the preprint server for biology · 2026Article
- Orthogonally Dispersed Spectroscopic Single-Molecule Localization Microscopy.Nanophotonics (Berlin, Germany) · 2026Article
- DeepQR: single-molecule QR codes for optical gene-expression analysis.Nanophotonics (Berlin, Germany) · 2025Article
- Deep learning-based spectroscopic single-molecule localization microscopy.Journal of biomedical optics · 2024Article
- Physically informed Monte Carlo simulation of dual-wedge prism-based spectroscopic single-molecule localization microscopy.Journal of biomedical optics · 2024Article
- Spectral precision improvement with demagnifying spectral images in spectroscopic nanoscopy.Journal of the Optical Society of America. A, Optics, image science, and vision · 2024Article
- Switchable and Functional Fluorophores for Multidimensional Single-Molecule Localization Microscopy.Chemical & biomedical imaging · 2023Review
- Spectroscopic single-molecule localization microscopy: applications and prospective.Nano convergence · 2023Review
- Enabling Spectrally Resolved Single-Molecule Localization Microscopy at High Emitter Densities.Nano letters · 2022Article
- Monolithic dual-wedge prism-based spectroscopic single-molecule localization microscopy.Nanophotonics (Berlin, Germany) · 2022Article
- Multimodal single-molecule microscopy with continuously controlled spectral resolution.Biophysical reports · 2021Article
- Improving spatial precision and field-of-view in wavelength-tagged single-particle tracking using spectroscopic single-molecule localization microscopy.Applied optics · 2021Article
- Investigating Single-Molecule Fluorescence Spectral Heterogeneity of Rhodamines Using High-Throughput Single-Molecule Spectroscopy.The journal of physical chemistry letters · 2021Article
- Super-resolution imaging of flat-mounted whole mouse cornea.Experimental eye research · 2021Article
- RainbowSTORM: an open-source ImageJ plug-in for spectroscopic single-molecule localization microscopy (sSMLM) data analysis and image reconstruction.Bioinformatics (Oxford, England) · 2020Article
- Sub-10-nm distance measurements between fluorophores using photon-accumulation enhanced reconstruction (PACER).Advanced photonics research · 2020Article
- Accelerating multicolor spectroscopic single-molecule localization microscopy using deep learning.Biomedical optics express · 2020Article
- Super-Resolution Imaging of Self-Assembled Nanocarriers Using Quantitative Spectroscopic Analysis for Cluster Extraction.Langmuir : the ACS journal of surfaces and colloids · 2020Article
- Symmetrically dispersed spectroscopic single-molecule localization microscopy.Light, science & applications · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
Abstract
Spectroscopic single-molecule localization microscopy (sSMLM) is a novel super-resolution imaging technology, which simultaneously records the nanoscopic location and the corresponding full emission spectrum of every stochastic single-molecule emission event. This spectroscopic imaging capability of sSMLM necessitates the establishment of a theoretical foundation of the newly introduced spectral precision and to guide the system design and optimization. Based on numerical simulation and analytical solution, we introduced such a theoretical model to analyze spectral precision by considering the main system parameters, including signal and background shot noises, readout noise, and the spectral calibration procedure. Using this model, we demonstrated the delicate balance among these parameters in achieving the optimal spectral precision and discovered that the best spectral precision can only be achieved at a particular system spectral dispersion. For example, with a given signal of 3000 photons and a readout noise of 2 e-, a system spectral dispersion of 1.6 nm/pixel is required for sSMLM to achieve the highest spectral precision of 1.31 nm.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.