ArticleJournal of immunology (Baltimore, Md. : 1950)2019
Inflammasome and Caspase-1 Activity Characterization and Evaluation: An Imaging Flow Cytometer-Based Detection and Assessment of Inflammasome Specks and Caspase-1 Activation.
Article in Journal of immunology (Baltimore, Md. : 1950), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Inflammasomes meet organoids and artificial intelligence: unraveling the complexity of gynecological inflammation.Frontiers in immunology · 2026Review
- Single-cell analysis reveals cell death as driver of NLRP3-mediated secretion of IL-1β in human monocytes.Nature immunology · 2025Article
- Atomic mechanisms of full-length ASC-mediated inflammasome assembly.Nature communications · 2025Article
- Comparative Analysis of Canonical Inflammasome Activation by Flow Cytometry, Imaging Flow Cytometry and High-Content Imaging.Inflammation · 2025Article
- A combined AI and cell biology approach surfaces targets and mechanistically distinct Inflammasome inhibitors.iScience · 2024Article
- Inherently Reduced Expression of ASC Restricts Caspase-1 Processing in Hepatocytes and Promotes Plasmodium Infection.Journal of immunology (Baltimore, Md. : 1950) · 2024Article
- Oligomeric states of ASC specks regulate inflammatory responses by inflammasome in the extracellular space.Cell death discovery · 2023Article
- Flow Imaging of the Inflammasome: Evaluating ASC Speck Characteristics and Caspase-1 Activity.Methods in molecular biology (Clifton, N.J.) · 2023Article
- What are NLRP3-ASC specks? an experimental progress of 22 years of inflammasome research.Frontiers in immunology · 2023Review
- Methods to Measure NLR Oligomerization I: Size Exclusion Chromatography, Co-immunoprecipitation, and Cross-Linking.Methods in molecular biology (Clifton, N.J.) · 2023Article
- IL-1β Promotes Expansion of IL-33American journal of respiratory cell and molecular biology · 2022Article
- Unknown/enigmatic functions of extracellular ASC.Immunology · 2021Review
- Techniques to Study Inflammasome Activation and Inhibition by Small Molecules.Molecules (Basel, Switzerland) · 2021Review
- The ASC Speck and NLRP3 Inflammasome Function Are Spatially and Temporally Distinct.Frontiers in immunology · 2021Article
- Complementary regulation of caspase-1 and IL-1β reveals additional mechanisms of dampened inflammation in bats.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Biomarkers and Associated Immune Mechanisms for Early Detection and Therapeutic Management of Sepsis.Immune network · 2020Review
- NLRP3 Sensing of Diverse Inflammatory Stimuli Requires Distinct Structural Features.Frontiers in immunology · 2020Article
- Review
- Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).European journal of immunology · 2019Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Inflammasome dysregulation is a hallmark of various inflammatory diseases. Evaluating inflammasome-associated structures (ASC specks) and caspase-1 activity by microscopy is time consuming and limited by small sample size. The current flow cytometric method, time of flight inflammasome evaluation (TOFIE), cannot visualize ASC specks or caspase-1 activity, making colocalization studies of inflammasome components and enzymatic activity impossible. We describe a rapid, high-throughput, single-cell, fluorescence-based image analysis method utilizing the Amnis ImageStream
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.