Evidence map›Paper›PMID 30597773›Full record

ArticleMicrobiologyOpen2019

The use of clade-specific PCR assays to identify novel nitrilase genes from environmental isolates.

Tríona-Marie Dooley-Cullinane, Catherine O'Reilly, Bilal Aslam, David P Weiner, David O'Neill, Erica Owens, Denise O'Meara, Lee Coffey

Open access · goldAbstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in MicrobiologyOpen, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 7 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Tríona-Marie Dooley-CullinaneDepartment of Science, Waterford Institute of Technology, Waterford, Ireland.ORCID 0000-0001-8478-6247
Catherine O'ReillyMolecular Biotechnology and Biocatalysis Group, Pharmaceutical and Molecular Biotechnology Research Center, Waterford Institute of Technology, Waterford, Ireland.
Bilal AslamDepartment of Science, Waterford Institute of Technology, Waterford, Ireland.
David P WeinerBASF Enzymes LLC, San Diego, California.
David O'NeillDepartment of Science, Waterford Institute of Technology, Waterford, Ireland.
Erica OwensDepartment of Science, Waterford Institute of Technology, Waterford, Ireland.
Denise O'MearaDepartment of Science, Waterford Institute of Technology, Waterford, Ireland.
Lee CoffeyDepartment of Science, Waterford Institute of Technology, Waterford, Ireland.
Waterford Institute of Technology · IEBASF (United States) · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nitrilase enzymes (EC 3.5.5.1) are responsible for the direct hydration of nitriles to their corresponding carboxylic acids and ammonia. The utilization of nitrilase enzymes in biocatalysis toward bio-pharmaceuticals and industrial applications facilitates the move towards green chemistry. The body of research presented describes a novel clade-specific touchdown PCR protocol for the detection of novel nitrilase genes. The presented study identified partial sequences of 15 novel nitrilase genes across 7 genera, with partial DNA sequence homology (%) displayed across an additional 16 genera. This research will prove valuable in the screening of microorganisms for the identification of novel clade-specific nitrilase genes, with predicted enantioselective profiles as determined by their clade characterizations.

Indexed as

Environmental MicrobiologyBacteriaBacterial ProteinsBase SequenceBiocatalysisCarboxylic AcidsCloning, MolecularHydro-LyasesNitrilesPhylogenyPolymerase Chain ReactionSequence Homology, Nucleic AcidSpecies SpecificityBacterial ProteinsCarboxylic AcidsHydro-Lyasesnitrile hydrataseNitrilesclade specificdegenerate primersenvironmental isolatesgene detectionnitrilasetouchdown PCR

Identifiers

PMID30597773
PMCPMC6460282
OpenAlexW2908021708

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.