ArticleBiophysics reports2018
Proteomic analysis of insulin secretory granules in INS-1 cells by protein correlation profiling.
Article in Biophysics reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 22 citations in OpenAlex.
- Stem cell-derived exosomes preserve diabetic islet function: a systematic review and meta-analysis of preclinical studies.Biophysics reports · 2026Article
- Dielectrophoresis reveals stimulus-induced remodeling of insulin granule subpopulations.Biophysical journal · 2026Article
- A Syd and RUFY dynein adaptor complex mediates axonal circulation of dense core vesicles.The Journal of cell biology · 2026Article
- Secretory stimuli distinctly regulate insulin secretory granule maturation through structural remodeling.Structure (London, England : 1993) · 2025Article
- Dielectrophoresis Reveals Stimulus-Induced Remodeling of Insulin Granule Subpopulations.bioRxiv : the preprint server for biology · 2025Article
- VAMP8 Is an Endosomal v-SNARE That Supports GLP-1 Receptor Recycling in Pancreatic β-Cells.Diabetes · 2025Article
- State of the Art of Bioengineering Approaches in Beta-Cell Replacement.Current transplantation reports · 2025Review
- Optimized Proteomic Analysis of Insulin Granules From MIN6 Cells Identifies Scamp3, a Novel Regulator of Insulin Secretion and Content.Diabetes · 2024Article
- Article
- Secretory Conservation in Insulin Producing Cells: Is There a System-Level Law of Mass Action in Biology?ACS omega · 2023Article
- Secretory autophagy promotes RAB37-mediated insulin secretion under glucose stimulation bothAutophagy · 2023Article
- VAMP4 regulates insulin levels by targeting secretory granules to lysosomes.The Journal of cell biology · 2022Article
- Islet organoid as a promising model for diabetes.Protein & cell · 2022Review
- The miR-216/miR-217 Cluster Regulates Lipid Metabolism in Laying Hens With Fatty Liver SyndromeFrontiers in veterinary science · 2022Article
- WFS1 functions in ER export of vesicular cargo proteins in pancreatic β-cells.Nature communications · 2021Article
- Isolation and Proteomics of the Insulin Secretory Granule.Metabolites · 2021Review
- The Pancreatic β-Cell: The Perfect Redox System.Antioxidants (Basel, Switzerland) · 2021Review
- A sensitive S-Trap-based approach to the analysis of T cell lipid raft proteome.Journal of lipid research · 2020Article
- In silico approach to predict pancreatic β-cells classically secreted proteins.Bioscience reports · 2020Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
abstractInsulin secretory granules (ISGs), a group of distinguishing organelles in pancreatic β cells, are responsible for the storage and secretion of insulin to maintain blood glucose homeostasis. The molecular mechanisms of ISG biogenesis, maturation, transportation, and exocytosis are still largely unknown because the proteins involved in these distinct steps have not been fully identified. Subcellular fractionation by density gradient centrifugation has been successfully employed to analyze the proteomes of numerous organelles. However, use of this method to elucidate the ISG proteome is limited by co-fractionated contaminants because ISGs are very dynamic and have abundant exchanges or contacts with other organelles, such as the Golgi apparatus, lysosomes, and endosomes. In this study, we developed a new strategy for identifying ISG proteins by protein correlation profiling (PCP)-based proteomics, which included ISG purification by OptiPrep density gradient centrifugation, label-free quantitative proteome, and identification of ISG proteins by correlating fractionation profiles between candidates and known ISG markers. Using this approach, we were able to identify 81 ISG proteins. Among them, TM9SF3, a nine-transmembrane protein, was considered a high confidence ISG candidate protein highlighted in the PCP network. Further biochemical and immunofluorescence assays indicated that TM9SF3 localized in ISGs, suggesting that it is a potential new ISG marker. GRAPHICAL ABSTRACT:
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.