ArticleBMC genomics2018
Female-specific SNP markers provide insights into a WZ/ZZ sex determination system for mud crabs Scylla paramamosain, S. tranquebarica and S. serrata with a rapid method for genetic sex identification.
Article in BMC genomics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Genome resequencing reveals an XX/XY sex determination system and male-specific markers iniScience · 2026Article
- Whole-Genome Resequencing Analysis Reveals Insights into Sex Determination and Gene Loci Associated with Sex Differences inInternational journal of molecular sciences · 2026Article
- Unlocking Demography: Developing an eDNA-Based Toolkit to Measure Sex Ratios From Populations.Molecular ecology resources · 2026Article
- Whole-Genome Resequencing Provides Novel Insights Into the Genetic Diversity, Population Structure, and Patterns of Runs of Homozygosity in Mud Crab (Evolutionary applications · 2025Article
- Unveiling potential sex-determining genes and sex-specific markers in autotetraploid Carassius auratus.Science China. Life sciences · 2024Article
- Cytogenetic evidence and dmrt linkage indicate male heterogamety in a non-bilaterian animal.PloS one · 2023Article
- Article
- Insights into chromosomal evolution and sex determination of Pseudobagrus ussuriensis (Bagridae, Siluriformes) based on a chromosome-level genome.DNA research : an international journal for rapid publication of reports on genes and genomes · 2022Article
- Sex determination mechanisms and sex control approaches in aquaculture animals.Science China. Life sciences · 2022Review
- Exploration of an XX/XY Sex Determination System and Development of PCR-Based Sex-specific Markers inFrontiers in genetics · 2022Article
- Vital Carbohydrate and Lipid Metabolites in Serum Involved in Energy Metabolism during Pubertal Molt of Mud Crab (Metabolites · 2021Article
- Thermal Tolerance and Physiological Changes in Mud Crab,Animals : an open access journal from MDPI · 2021Article
- Evidence of Sex Differentiation Based on Morphological Traits During the Early Development Stage of Mud CrabFrontiers in veterinary science · 2021Article
- Identification of Sex-Specific Markers Through 2b-RAD Sequencing in the Sea Urchin (Frontiers in genetics · 2021Article
- Application of SNP in Genetic Sex Identification and Effect of Estradiol on Gene Expression of Sex-Related Genes inFrontiers in endocrinology · 2021Article
- Article
- Transcriptome analysis of genes and pathways associated with metabolism in Scylla paramamosain under different light intensities during indoor overwintering.BMC genomics · 2020Article
- Identification of Y chromosome markers in the eastern three-lined skink (Bassiana duperreyi) using in silico whole genome subtraction.BMC genomics · 2020Article
- Harnessing genomics to fast-track genetic improvement in aquaculture.Nature reviews. Genetics · 2020Review
- High-Density Genetic Linkage Maps Provide Novel Insights Into ZW/ZZ Sex Determination System and Growth Performance in Mud Crab (Frontiers in genetics · 2019Article
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15 authors.
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Abstract
backgroundMud crabs, Scylla spp., are commercially important large-size marine crustaceans in the Indo-West Pacific region. As females have the higher growth rate and economic value, the production of all female stocks is extremely essential in aquaculture. However, the sex determination mechanism is still unclear. Development of sex-specific genetic markers based on next-generation sequencing proved to be an effective tool for discovering sex determination system in various animals.
resultsRestriction-site associated DNA sequencing (RAD-seq) was employed to isolate sex-specific SNP markers for S. paramamosain. A total of 335.6 million raw reads were obtained from 20 individuals, of which 204.7 million were from 10 females and 130.9 million from 10 males. After sequence assembly and female-male comparison, 20 SNP markers were identified to be sex-specific. Furthermore, ten SNPs in a short sequence (285 bp) were confirmed heterozygous in females and homozygous in males in a large population by PCR amplification and sequencing. Subsequently, a female-specific primer was successfully designed according to the female-specific nucleotide which could amplify an expected band from females but not from males. Thus, a rapid and effective method for molecular sexing in S. paramamosain was developed, meanwhile, this method could successfully identify the sex of S. tranquebarica and S. serrata. Finally, nine and four female-specific SNP markers were detected in S. tranquebarica and S. serrata, respectively.
conclusionsSex-specific SNP markers were firstly identified in crab species and showed female heterogamety and male homogamety, which provided strong genetic evidence for a WZ/ZZ sex determination system in mud crabs S. paramamosain, S. tranquebarica and S. serrata. These findings will lay a solid foundation for the study of sex determination mechanism, sex chromosome evolution, and the development of mono-sex population in crustaceans.
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