ArticleACS biomaterials science & engineering2018
Organotypic 3D Culture in Nanoscaffold Microwells Supports Salivary Gland Stem-Cell-Based Organization.
Article in ACS biomaterials science & engineering, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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Who cites it
23 citing papers in PubMed, 54 citations in OpenAlex.
- Cryoelectrospun Elastin-Alginate Scaffolds Support In Vitro 3D Epithelial-Stromal Cocultures for Salivary Tissue Engineering.Gels (Basel, Switzerland) · 2025Article
- Extracellular vesicles derived from salivary gland stem cells cultured on microwell scaffolds loaded with WNT3A promote the recovery of salivary gland function damaged by radiation via the YWHAZ-PI3K-AKT pathway.Bioactive materials · 2025Article
- Expansion of functional human salivary acinar cell spheroids with reversible thermo-ionically crosslinked 3D hydrogels.International journal of oral science · 2025Article
- Morphological profiling for drug discovery in the era of deep learning.Briefings in bioinformatics · 2024Review
- A microwell platform for high-throughput longitudinal phenotyping and selective retrieval of organoids.Cell systems · 2023Article
- Salivary gland bioengineering - yesterday, today, tomorrow!Histology and histopathology · 2023Review
- Spatially Defined Cell-Secreted Protein Detection Using Granular Hydrogels: μGeLISA.ACS biomaterials science & engineering · 2023Article
- Engineered organoids in oral and maxillofacial regeneration.iScience · 2023Review
- Preparation and characterization of a novel triple composite scaffold containing silk fibroin, chitosan, extracellular matrix and the mechanism of Akt/FoxO signaling pathway in colonic cancer cells cultured in 3D.Frontiers in bioengineering and biotechnology · 2023Article
- Primary human organoids models: Current progress and key milestones.Frontiers in bioengineering and biotechnology · 2023Review
- Tissue-engineered edible bird's nests (TeeBN).International journal of bioprinting · 2023Article
- Cocktail Formula and Application Prospects for Oral and Maxillofacial Organoids.Tissue engineering and regenerative medicine · 2022Review
- Advances of Engineered Hydrogel Organoids within the Stem Cell Field: A Systematic Review.Gels (Basel, Switzerland) · 2022Review
- Bioengineering in salivary gland regeneration.Journal of biomedical science · 2022Review
- Encapsulation of Primary Salivary Gland Acinar Cell Clusters and Intercalated Ducts (AIDUCs) within Matrix Metalloproteinase (MMP)-Degradable Hydrogels to Maintain Tissue Structure and Function.Advanced healthcare materials · 2022Article
- Engineering Hydrogels for the Development of Three-Dimensional In Vitro Models.International journal of molecular sciences · 2022Review
- Bioengineering Approaches for the Advanced Organoid Research.Advanced materials (Deerfield Beach, Fla.) · 2021Review
- A matrigel-free method to generate matured human cerebral organoids using 3D-Printed microwell arrays.Bioactive materials · 2021Article
- Development of a functional salivary gland tissue chip with potential for high-content drug screening.Communications biology · 2021Article
- Trimers Conjugated to Fibrin Hydrogels Promote Salivary Gland Function.Journal of dental research · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The self-organizing properties of stem cells have been exploited to generate organoids, organ-specific, cell-containing, three-dimensional (3D) structures. The present study aimed to introduce a novel bioengineering technique for driving the effective organization of adult tissue stem cells via niche-independent 3D microwell culture. Microwells were fabricated by photopatterning poly(ethylene glycol) hydrogel in the presence of an electrospun polycaprolactone nanofibrous scaffold. Human single clonal salivary gland stem cells (SGSCs) were cultured in nanofibrous microwells through two simple steps, priming and differentiation. Before the induction of 3D organization, single clonal SGSCs were preconditioned to aggregate to form 3D spheroids in different matrices, such as Matrigel, floating dish, and microwells. Expression of salivary stem cell markers and pluripotency markers was greater in 3D spheroid cultures than in 2D plastic culture. Lobular structures were organized by changing media, and those in microwells exhibited higher salivary acinar, ductal, and tight junction marker levels and decreased stem-cell marker levels relative to other 3D cultures. Furthermore, higher α-amylase secretion and intracellular calcium levels were observed in the presence of adrenergic or cholinergic agonists, respectively, along with an increased intracellular pH, suggesting more functional salivary organoid formation. These microwell-driven organoids also engrafted successfully into nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice. Our results showed that microwell-cultured SGSCs organize into salivary structures and that this biomimetic 3D culture technique can promote effective generation of niche-independent single stem-cell-based 3D organoids.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.