Evidence map›Paper›PMID 30590035›Full record

ArticleCell reports2018

The TFAP2C-Regulated OCT4 Naive Enhancer Is Involved in Human Germline Formation.

Di Chen, Wanlu Liu, Jill Zimmerman, William A Pastor, Rachel Kim, Linzi Hosohama, Jamie Ho, Marianna Aslanyan, Joanna J Gell, Steven E Jacobsen and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.

0numbers the graph read from it
0cells of the map it votes in
52citing papers in PubMed
3.2field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

52 citing papers in PubMed, 89 citations in OpenAlex.

  1. Article
  2. Article
  3. Mechanisms of human germ cell development.Nature reviews. Molecular cell biology · 2026
    Review
  4. Article
  5. Article
  6. Review
  7. Article
  8. Review
  9. Article
  10. Advances inFrontiers in endocrinology · 2025
    Review
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Review
  17. Article
  18. DMRT1 drives the human germline forward.Nature cell biology · 2023
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Di ChenDepartment of Molecular Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, USA.
Wanlu LiuMolecular Biology Institute, University of California, Los Angeles, Los Angeles, CA, USA.
Jill ZimmermanDepartment of Molecular Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, USA.
William A PastorDepartment of Molecular Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, USA.
Rachel KimEli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA, USA.
Linzi HosohamaDepartment of Molecular Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, USA.
Jamie HoDepartment of Molecular Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, USA.
Marianna AslanyanDepartment of Molecular Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, USA.
Joanna J GellDepartment of Pediatrics, Division of Hematology-Oncology, Los Angeles, CA 90095, USA; David Geffen School of Medicine, Los Angeles, CA 90095, USA.
Steven E JacobsenDepartment of Molecular Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, USA; Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA, USA; Department of Biological Chemistry, University of California, Los Angeles, Los Angeles, CA, USA; Howard Hughes Medical Institute, University of California, Los Angeles, Los Angeles, CA, USA.
Amander T ClarkDepartment of Molecular Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, USA; Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA, USA; Jonsson Comprehensive Cancer Center, University of California, Los Angeles, Los Angeles, CA, USA. Electronic address: clarka@ucla.edu.
University of California, Los Angeles · USHoward Hughes Medical Institute · US

Funding

LABORATORY OF DEVELOPMENTAL BIOLOGYR24HD000836 · NICHD · UNIVERSITY OF WASHINGTON · PI Ian Amos Glass · 1995 to 2026
$16.9M
Differentiating embryonic stem cells into developing germ lineR01HD079546 · NICHD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CLARK, AMANDER · 2015 to 2024
$3.7M
NICHD NIH HHS R01 HD079546NICHD NIH HHS R24 HD000836
6 · The paper itself

Abstract

Human primordial germ cells (hPGCs) are the first embryonic progenitors in the germ cell lineage, yet the molecular mechanisms required for hPGC formation are not well characterized. To identify regulatory regions in hPGC development, we used the assay for transposase-accessible chromatin using sequencing (ATAC-seq) to systematically characterize regions of open chromatin in hPGCs and hPGC-like cells (hPGCLCs) differentiated from human embryonic stem cells (hESCs). We discovered regions of open chromatin unique to hPGCs and hPGCLCs that significantly overlap with TFAP2C-bound enhancers identified in the naive ground state of pluripotency. Using CRISPR/Cas9, we show that deleting the TFAP2C-bound naive enhancer at the OCT4 locus (also called POU5F1) results in impaired OCT4 expression and a negative effect on hPGCLC identity.

Indexed as

Gene Expression Regulation, DevelopmentalChromatinEnhancer Elements, GeneticFemaleGerm CellsHumansKruppel-Like Factor 4Kruppel-Like Transcription FactorsMaleNucleotide MotifsOctamer Transcription Factor-3Pluripotent Stem CellsTranscription Factor AP-2TranscriptomeChromatinKruppel-Like Factor 4Kruppel-Like Transcription FactorsOctamer Transcription Factor-3POU5F1 protein, humanTFAP2C protein, humanTranscription Factor AP-2enhancernaiveOCT4PGCPGCLCpluripotencyTFAP2C

Identifiers

PMID30590035
PMCPMC6342560
OpenAlexW2906273947

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.