Evidence map›Paper›PMID 30589194›Full record

ArticleChembiochem : a European journal of chemical biology2019

Identifying the Biosynthetic Gene Cluster for Triacsins with an N-Hydroxytriazene Moiety.

Frederick F Twigg, Wenlong Cai, Wei Huang, Joyce Liu, Michio Sato, Tynan J Perez, Jiaxin Geng, Moriel J Dror, Ismael Montanez, Tate L Tong and 2 more

Abstract read
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In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Chemical science · 2023
    Article
  7. Article
  8. Biosynthesis of Isonitrile- and Alkyne-Containing Natural Products.Annual review of chemical and biomolecular engineering · 2022
    Review
  9. Review
  10. Review
  11. Article
  12. Article
  13. Article
  14. Review
  15. The l-Alanosine Gene Cluster Encodes a Pathway for Diazeniumdiolate Biosynthesis.Chembiochem : a European journal of chemical biology · 2020
    Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Frederick F TwiggDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, 2151 Berkeley Way, Berkeley, CA, 94704, USA.ORCID 0000-0003-4074-4218
Wenlong CaiDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, 2151 Berkeley Way, Berkeley, CA, 94704, USA.
Wei HuangDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, 2151 Berkeley Way, Berkeley, CA, 94704, USA.
Joyce LiuDepartment of Bioengineering, University of California, Berkeley, 2151 Berkeley Way, Berkeley, CA, 94704, USA.
Michio SatoDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, 2151 Berkeley Way, Berkeley, CA, 94704, USA.
Tynan J PerezDepartment of Chemistry, University of California, Berkeley, 2151 Berkeley Way, Berkeley, CA, 94704, USA.
Jiaxin GengDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, 94704, USA.
Moriel J DrorDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, 2151 Berkeley Way, Berkeley, CA, 94704, USA.
Ismael MontanezDepartment of Chemistry, University of California, Berkeley, 2151 Berkeley Way, Berkeley, CA, 94704, USA.
Tate L TongDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, 2151 Berkeley Way, Berkeley, CA, 94704, USA.
Hyunsu LeeDepartment of Chemistry, University of California, Berkeley, 2151 Berkeley Way, Berkeley, CA, 94704, USA.
Wenjun ZhangDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, 2151 Berkeley Way, Berkeley, CA, 94704, USA.ORCID 0000-0001-9348-972X

Funding

In Situ Natural Product Labeling and ApplicationsDP2AT009148 · NCCIH · UNIVERSITY OF CALIFORNIA BERKELEY · PI ZHANG, WENJUN · 2015 to 2020
$2.8M
NCCIH NIH HHS DP2 AT009148
6 · The paper itself

Abstract

Triacsins are a family of natural products having in common an N-hydroxytriazene moiety not found in any other known secondary metabolites. Though many studies have examined the biological activity of triacsins in lipid metabolism, their biosynthesis has remained unknown. Here we report the identification of the triacsin biosynthetic gene cluster in Streptomyces aureofaciens ATCC 31442. Bioinformatic analysis of the gene cluster led to the discovery of the tacrolimus producer Streptomyces tsukubaensis NRRL 18488 as a new triacsin producer. In addition to targeted gene disruption to identify necessary genes for triacsin production, stable isotope feeding was performed in vivo to advance the understanding of N-hydroxytriazene biosynthesis.

Indexed as

Multigene FamilyComputational BiologyEnzyme InhibitorsEnzymesGenes, BacterialMutationStreptomycesStreptomyces aureofaciensTriazenesEnzyme InhibitorsEnzymesTriazenesacyl-CoA synthetase inhibitorsbiocatalysisbiosynthesisnitrous acidN−N bonds

Identifiers

PMID30589194
PMCPMC6590916

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.