Evidence map›Paper›PMID 30588784›Full record

ArticleThe world journal of men's health2020

New Insights on the Mechanisms Affecting Fertility in Men with Non-Seminoma Testicular Cancer before Cancer Therapy.

Tania R Dias, Ashok Agarwal, Peter N Pushparaj, Gulfam Ahmad, Rakesh Sharma

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The world journal of men's health, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 18 citations in OpenAlex.

  1. Review
  2. Proteostasis as a Sentry for Sperm Quality and Male Fertility.Advances in experimental medicine and biology · 2025
    Review
  3. Whole Mitochondrial Genome Sequencing Analysis of Canine Testicular Tumours.International journal of molecular sciences · 2024
    Article
  4. Article
  5. Article
  6. Review
  7. Semen quality from patients affected by seminomatous and non-seminomatous testicular tumor.International braz j urol : official journal of the Brazilian Society of Urology
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 4 countries.

Tania R DiasAmerican Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA.ORCID https://orcid.org/0000-0002-2336-8006
Ashok AgarwalAmerican Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA. agarwaa@ccf.org.ORCID https://orcid.org/0000-0003-0585-1026
Peter N PushparajCenter of Excellence in Genomic Medicine Research, Faculty of Applied Medical Sciences, Jeddah, Saudi Arabia.ORCID https://orcid.org/0000-0001-7574-1880
Gulfam AhmadDivision of Pathology, School of Medical Sciences, Sydney University, Sydney, Australia.ORCID https://orcid.org/0000-0002-4225-8347
Rakesh SharmaAmerican Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA.ORCID https://orcid.org/0000-0002-9664-6978
Cleveland Clinic · USThe University of Sydney · AUUniversity of Jeddah · SA

Funding

Cleveland ClinicFulbright Program E0585639Fundação para a Ciência e a Tecnologia SFRH/BD/109284/2015
6 · The paper itself

Abstract

purposePatients with non-seminoma testicular cancer (NSTC) cancer can be subfertile or infertile, and present reduced sperm quality, but the underlying mechanisms are unknown. The aim of this study was to compare the sperm proteome of patients with NSTC, who cryopreserved their sperm before starting cancer treatment, with that from healthy fertile men. MATERIALS AND

methodsSemen volume, sperm motility and sperm concentration were evaluated before the cryopreservation of samples from patients with NSTC (n=15) and the control group (n=15). Sperm proteomic analysis was performed by liquid chromatography-tandem mass spectrometry and the differentially expressed proteins (DEPs) between the two groups were identified using bioinformatic tools.

resultsA total of 189 DEPs was identified in the dataset, from which five DEPs related to sperm function and fertilization were selected for validation by Western blot. We were able to validate the underexpression of the mitochondrial complex subunits NADH:Ubiquinone Oxidoreductase Core Subunit S1 (NDUFS1) and ubiquinol-cytochrome C reductase core protein 2 (UQCRC2), as well as the underexpression of the testis-specific sodium/potassium-transporting ATPase subunit alpha-4 (ATP1A4) in the NSTC group.

conclusionsOur results indicate that sperm mitochondrial dysfunction may explain the observed decrease in sperm concentration, total sperm count and total motile count in NSTC patients. The identified DEPs may serve as potential biomarkers for the pathophysiology of subfertility/infertility in patients with NSTC. Our study also associates the reduced fertilizing ability of NSTC patients with the dysregulation of important sperm molecular mechanisms.

Indexed as

Cancer therapyMale fertilityNon-seminomaSperm qualityTesticular cancer

Identifiers

PMID30588784
PMCPMC7076305
OpenAlexW2906509019

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.