ArticleGenes, brain, and behavior2019
Novel zebrafish behavioral assay to identify modifiers of the rapid, nongenomic stress response.
Article in Genes, brain, and behavior, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 67 citations in OpenAlex.
- Multi-ancestry whole genome sequencing analysis of lean body mass.Genome biology · 2025Pooled it
- Impacts of variation in experimental parameters on larval zebrafish behavior: Enhancing rigor and reproducibility in neurobehavioral research.Behavioural processes · 2026Article
- Disrupting fzd9b in Zebrafish Recapitulates Stress- and Anxiety-Like Behaviours Relevant to Williams Syndrome.Molecular neurobiology · 2026Article
- Zebrafish exposed to a cocktail of pesticides during early development display long-lasting neurobehavioral alterations.Archives of toxicology · 2025Article
- Acute Stress Effects over Time on Gene Expression Patterns in the Male Zebrafish (Animals : an open access journal from MDPI · 2025Article
- Zebrafish as a Model Organism for Post-Traumatic Stress Disorder: Insights into Stress Mechanisms and Behavioral Assays.Biology · 2025Review
- doc2a and doc2b contribute to locomotor and social behaviors by down-regulating npas4b in zebrafish.BMC biology · 2025Article
- Zebrafishology, study design guidelines for rigorous and reproducible data using zebrafish.Communications biology · 2025Review
- Exploring the Toxicity and Therapeutic Potential ofInternational journal of molecular sciences · 2025Article
- Marigold: a machine learning-based web app for zebrafish pose tracking.BMC bioinformatics · 2025Article
- Assessing the effects of fluorine-free and PFAS-containing firefighting foams on development and behavioral responses using a zebrafish-based platform.Chemosphere · 2024Article
- Upregulation of coagulation factor V by glucocorticoid in the preovulatory follicles of zebrafish.The Journal of steroid biochemistry and molecular biology · 2024Article
- Jnk1 and downstream signalling hubs regulate anxiety-like behaviours in a zebrafish larvae phenotypic screen.Scientific reports · 2024Article
- Elevated glucocorticoid alters the developmental dynamics of hypothalamic neurogenesis in zebrafish.Communications biology · 2024Article
- Key HPI axis receptors facilitate light adaptive behavior in larval zebrafish.Scientific reports · 2024Article
- Exploring the Hawaiian Ala Wai Watershed with Zebrafish.Zebrafish · 2024Article
- Multiple faces of stress in the zebrafish (Frontiers in physiology · 2024Article
- Article
- Advances in Zebrafish as a Comprehensive Model of Mental Disorders.Depression and anxiety · 2023Review
- Improving zebrafish laboratory welfare and scientific research through understanding their natural history.Biological reviews of the Cambridge Philosophical Society · 2022Review
Corrections and comments
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Authors and funding
14 authors at 1 institution in 1 country.
Funding
Abstract
When vertebrates face acute stressors, their bodies rapidly undergo a repertoire of physiological and behavioral adaptations, which is termed the stress response. Rapid changes in heart rate and blood glucose levels occur via the interaction of glucocorticoids and their cognate receptors following hypothalamic-pituitary-adrenal axis activation. These physiological changes are observed within minutes of encountering a stressor and the rapid time domain rules out genomic responses that require gene expression changes. Although behavioral changes corresponding to physiological changes are commonly observed, it is not clearly understood to what extent hypothalamic-pituitary-adrenal axis activation dictates adaptive behavior. We hypothesized that rapid locomotor response to acute stressors in zebrafish requires hypothalamic-pituitary-interrenal (HPI) axis activation. In teleost fish, interrenal cells are functionally homologous to the adrenocortical layer. We derived eight frameshift mutants in genes involved in HPI axis function: two mutants in exon 2 of mc2r (adrenocorticotropic hormone receptor), five in exon 2 or 5 of nr3c1 (glucocorticoid receptor [GR]) and two in exon 2 of nr3c2 (mineralocorticoid receptor [MR]). Exposing larval zebrafish to mild environmental stressors, acute changes in salinity or light illumination, results in a rapid locomotor response. We show that this locomotor response requires a functioning HPI axis via the action of mc2r and the canonical GR encoded by nr3c1 gene, but not MR (nr3c2). Our rapid behavioral assay paradigm based on HPI axis biology can be used to screen for genetic and environmental modifiers of the hypothalamic-pituitary-adrenal axis and to investigate the effects of corticosteroids and their cognate receptor interactions on behavior.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.