ArticlePlant reproduction2019
Endosperm-specific transcriptome analysis by applying the INTACT system.
Article in Plant reproduction, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Molecular understanding of plant pollination, fertilization, seed development and protoplast regeneration.Science China. Life sciences · 2026Review
- TIE1 acts as a maternal brake on fertilization-independent endosperm development by associating with PRC2 to enforce imprinting.Nature plants · 2026Article
- Dissecting the cellular architecture and genetic circuitry of the soybean seed.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Epigenetic and transcriptional consequences in the endosperm of chemically induced transposon mobilization in Arabidopsis.Nucleic acids research · 2024Article
- Parental conflict driven regulation of endosperm cellularization by a family of Auxin Response Factors.Nature plants · 2024Article
- Review
- Spatial and temporal regulation of parent-of-origin allelic expression in the endosperm.Plant physiology · 2023Article
- DNA methylation dynamics during germline development.Journal of integrative plant biology · 2022Review
- Bypassing reproductive barriers in hybrid seeds using chemically induced epimutagenesis.The Plant cell · 2022Article
- Deciphering Precise Gene Transcriptional Expression Using gwINTACT in Tomato.Frontiers in plant science · 2022Article
- Current status and trends in forest genomics.Forestry research · 2022Review
- Endosperm-Embryo Communications: Recent Advances and Perspectives.Plants (Basel, Switzerland) · 2021Review
- INT-Hi-C reveals distinct chromatin architecture in endosperm and leaf tissues of Arabidopsis.Nucleic acids research · 2021Article
- FlsnRNA-seq: protoplasting-free full-length single-nucleus RNA profiling in plants.Genome biology · 2021Article
- Meiocyte Isolation by INTACT and Meiotic Transcriptome Analysis inFrontiers in plant science · 2021Article
- Combining GAL4 GFP enhancer trap with split luciferase to measure spatiotemporal promoter activity in Arabidopsis.The Plant journal : for cell and molecular biology · 2020Article
- Big data from small tissues: extraction of high-quality RNA for RNA-sequencing from different oilseedPlant methods · 2020Article
- Genomic imprinting in plants-revisiting existing models.Genes & development · 2020Review
- Article
- Special issue: cellular omics methods in plant reproduction research.Plant reproduction · 2019Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
key messageWe report the adaptation of the INTACT method for RNA-sequencing in the endosperm and demonstrate its feasibility for allele-specific expression analysis. Tissue-specific transcriptome analyses provide important insights into the developmental programs of defined cell types. The isolation of nuclei tagged in specific cell types (INTACT) is a versatile method that allows to isolate highly pure nuclei from defined tissue types that can be used for several downstream applications. Here, we describe the adaptation of INTACT from endosperm nuclei for high-throughput RNA-sequencing. By analyzing the ratio of parental reads and tissue-specific gene expression in the endosperm, we could assess the contamination level of our samples. Based on this analysis, we estimate that in most of the samples the contamination level is lower than in previously published datasets. We further show that the nuclear transcriptome and total transcriptome of the endosperm are well correlated. Together, our data show that INTACT of the endosperm is a reliable methodology for endosperm-specific transcriptome analysis that overcomes the limitation of time-consuming manual endosperm dissection that is connected with high levels of maternal tissue contamination. INTACT does not rely on expensive equipment and can be set up in every standard molecular biology laboratory, making it the method of choice for future molecular studies of the endosperm.
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