ArticleNPJ genomic medicine2018
TAC-seq: targeted DNA and RNA sequencing for precise biomarker molecule counting.
Article in NPJ genomic medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed.
- Biomarkers for Endometrial Receptivity: Implications for Infertility, Implantation Failure, and Advances in Diagnosis and Treatment.International journal of molecular sciences · 2026Review
- Investigating cycle-to-cycle variation and the impact of endometrial injury through differential gene expression analysis.Human reproduction (Oxford, England) · 2026Article
- High-throughput profiling of chemical-induced gene expression across 93,644 perturbations.Nature methods · 2025Article
- Factors influencing endometrial receptivity in women with recurrent implantation failure.BMC women's health · 2025Article
- Recent Technologies towards Diagnostic and Therapeutic Applications of Circulating Nucleic Acids in Colorectal Cancers.International journal of molecular sciences · 2024Review
- The endometrial transcriptome of infertile women with and without implantation failure.Acta obstetricia et gynecologica Scandinavica · 2024Article
- Single-Nucleus RNA-Seq: Open the Era of Great Navigation for FFPE Tissue.International journal of molecular sciences · 2023Review
- Targeted gene expression profiling for accurate endometrial receptivity testing.Scientific reports · 2023Article
- PHDs-seq: a large-scale phenotypic screening method for drug discovery through parallel multi-readout quantification.Cell regeneration (London, England) · 2023Article
- Liquid Biopsy for Lung Cancer: Up-to-Date and Perspectives for Screening Programs.International journal of molecular sciences · 2023Review
- TORNADO-seq: A Protocol for High-Throughput Targeted RNA-seq-Based Drug Screening in Organoids.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Commercially Available Molecular Approaches to Evaluate Endometrial Receptivity: A Systematic Review and Critical Analysis of the Literature.Diagnostics (Basel, Switzerland) · 2022Review
- Interaction between endometrial microbiota and host gene regulation in recurrent implantation failure.Journal of assisted reproduction and genetics · 2022Article
- Transcriptomics of receptive endometrium in women with sonographic features of adenomyosis.Reproductive biology and endocrinology : RB&E · 2022Observational
- Coupling miR/isomiR and mRNA Expression Signatures Unveils New Molecular Layers of Endometrial Receptivity.Life (Basel, Switzerland) · 2021Article
- Guidelines for biomarker discovery in endometrium: correcting for menstrual cycle bias reveals new genes associated with uterine disorders.Molecular human reproduction · 2021Article
- Article
- Prospective evaluation of NGS-based liquid biopsy in untreated late stage non-squamous lung carcinoma in a single institution.Journal of translational medicine · 2020Article
- Review
- Article
Corrections and comments
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Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Targeted next-generation sequencing (NGS) methods have become essential in medical research and diagnostics. In addition to NGS sensitivity and high-throughput capacity, precise biomolecule counting based on unique molecular identifier (UMI) has potential to increase biomolecule detection accuracy. Although UMIs are widely used in basic research its introduction to clinical assays is still in progress. Here, we present a robust and cost-effective TAC-seq (Targeted Allele Counting by sequencing) method that uses UMIs to estimate the original molecule counts of mRNAs, microRNAs, and cell-free DNA. We applied TAC-seq in three different clinical applications and compared the results with standard NGS. RNA samples extracted from human endometrial biopsies were analyzed using previously described 57 mRNA-based receptivity biomarkers and 49 selected microRNAs at different expression levels. Cell-free DNA aneuploidy testing was based on cell line (47,XX, +21) genomic DNA. TAC-seq mRNA profiling showed identical clustering results to transcriptome RNA sequencing, and microRNA detection demonstrated significant reduction in amplification bias, allowing to determine minor expression changes between different samples that remained undetermined by standard NGS. The mimicking experiment for cell-free DNA fetal aneuploidy analysis showed that TAC-seq can be applied to count highly fragmented DNA, detecting significant (
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.