Evidence map›Paper›PMID 30588012›Full record

ArticleOncoTargets and therapy2018

Resveratrol induces apoptosis in human melanoma cell through negatively regulating Erk/PKM2/Bcl-2 axis.

Hailong Zhao, Limin Han, Yi Jian, Yuntao Ma, Wanyue Yan, Xiaowen Chen, Haiyan Xu, Lijuan Li

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in OncoTargets and therapy, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
3.0field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 58 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Hailong ZhaoDepartment of Pathophysiology, Zunyi Medical University, Zunyi, Guizhou Province 563000, People's Republic of China, hailongzhao@zmc.edu.cn.
Limin HanDepartment of Pathophysiology, Zunyi Medical University, Zunyi, Guizhou Province 563000, People's Republic of China, hailongzhao@zmc.edu.cn.
Yi JianThe First Clinical Institute, The School of Medicine and Science, Zunyi Medical University, Zunyi, Guizhou Province 563000, People's Republic of China.
Yuntao MaThe First Clinical Institute, The Department of Clinical Medicine, Zunyi Medical University, Zunyi, Guizhou Province 563000, People's Republic of China.
Wanyue YanThe First Clinical Institute, The Department of Clinical Medicine, Zunyi Medical University, Zunyi, Guizhou Province 563000, People's Republic of China.
Xiaowen ChenDepartment of Pathophysiology, Zunyi Medical University, Zunyi, Guizhou Province 563000, People's Republic of China, hailongzhao@zmc.edu.cn.
Haiyan XuDepartment of Pathophysiology, Zunyi Medical University, Zunyi, Guizhou Province 563000, People's Republic of China, hailongzhao@zmc.edu.cn.
Lijuan LiDepartment of Pathophysiology, Zunyi Medical University, Zunyi, Guizhou Province 563000, People's Republic of China, hailongzhao@zmc.edu.cn.
Zunyi Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundResveratrol is known as a natural phytoalexin found in grapes and wine, which has significant antitumor activity under in vitro and in vivo conditions. In recent years, great progress has been made in understanding the underlying mechanisms of resveratrol in inducing cellular apoptosis of melanoma cells. Our previous study has shown that the apoptosis regulation of resveratrol in melanoma cells was independent of activation of classical apoptosis-related protein p53. MATERIALS AND

methodsMTT assay and 5-bromo-2'-deoxyuridine staining assay were used to analyze cell viability and proliferation. Immunofluorescence analysis of γ-H2AX was employed to clarify DNA damages. Annexin V-propidine iodide/fluorescein isothiocyanate assay was performed to evaluate the cell apoptosis. The mechanisms underlying the activation of M2-type pyruvate kinase (PKM2) by Erk1/2 to stabilize and maintain Bcl-2 signaling was investigated by subcellular fractionation analyses, immunofluorescence analysis, co-immunoprecipitation assay, ubiquitination assay, and glutathione S-transferase pull-down assay.

resultsIn the present study, we found that resveratrol dramatically inhibited melanoma cell proliferation and induced cell apoptosis through upregulation of p53 in a concentration-dependent manner. Conversely, p53 downregulation by short hairpin RNA couldn't rescue resveratrol-induced cell proliferation inhibition or apoptosis enlargement. Additionally, we found that resveratrol downregulated antiapoptotic protein Bcl-2 and activated Bax in the protein levels by promoting Bcl-2 degradation and cytochrome c release. Moreover, we discovered that PKM2, had a key role in cell apoptosis triggered by resveratrol through interacting with Bcl-2. Based on these results, we overexpressed PKM2 in melanoma cells and found that this prevented resveratrol-induced apoptosis by stabilizing the protein level of Bcl-2.

conclusionTaken together, our results provided a novel mechanism accounting for the apoptosis induction of resveratrol in melanoma cells and suggested that downregulating Erk/PKM2/Bcl-2 axis appears to be a new approach for the prevention or treatment of melanoma.

Indexed as

antitumorcytochrome cER stressubiquitination

Identifiers

PMID30588012
PMCPMC6294058
OpenAlexW2903756779

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.