ArticleAnnals of clinical microbiology and antimicrobials2018
Metagenomic next-generation sequencing as a diagnostic tool for toxoplasmic encephalitis.
Article in Annals of clinical microbiology and antimicrobials, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The added value of metagenomic next-generation sequencing in central nervous system infections: a systematic review of case reports.Infection · 2025Pooled it
- Metagenomic and Targeted Next-Generation Sequencing in Infectious Disease Diagnostics: Current Applications, Challenges, and Future Perspectives.Diagnostics (Basel, Switzerland) · 2026Review
- Central Nervous System Toxoplasmosis is an Under-Recognized Opportunistic infection in Uganda.Journal of tropical medicine · 2026Article
- Construction of a preliminary digital parasite specimen database for parasitology education and research.Scientific reports · 2025Article
- Detection of toxoplasma tachyzoites in the cerebrospinal fluid of a COVID-19 positive SLE patient: a case study.BMC infectious diseases · 2025Article
- Comparison of mNGS with conventional methods for diagnosis of cryptococcal meningitis: a retrospective study.Scientific reports · 2025Article
- Toxoplasmosis in a case with multiple serous effusions and severe aplastic anemia.BMC infectious diseases · 2024Article
- Nanopore targeted sequencing-based diagnosis of central nervous system infections in HIV-infected patients.Annals of clinical microbiology and antimicrobials · 2024Article
- Application of metagenomic next-generation sequencing in the diagnosis of infectious diseases.Frontiers in cellular and infection microbiology · 2024Review
- Clinical value of macrogenome next-generation sequencing on infections.Open life sciences · 2024Article
- Development and Optimization of an Unbiased, Metagenomics-Based Pathogen Detection Workflow for Infectious Disease and Biosurveillance Applications.Tropical medicine and infectious disease · 2023Article
- Case report: A toxoplasmic encephalitis in an immunocompromised child detected through metagenomic next-generation sequencing.Frontiers in public health · 2023Article
- Next-generation sequencing: what are the needs in routine clinical microbiology? A survey among clinicians involved in infectious diseases practice.Frontiers in medicine · 2023Article
- Metagenomic next-generation sequencing for the etiological diagnosis of rabies virus in cerebrospinal fluid.Frontiers in medicine · 2023Article
- Where Have All the Diagnostic Morphological Parasitologists Gone?Journal of clinical microbiology · 2022Article
- Primary Toxoplasma gondii infection-associated with hemophagocytic syndrome in a man with HIV infection: a case report.BMC infectious diseases · 2022Article
- Direct Metagenomic Diagnosis of Community-Acquired Meningitis: State of the Art.Frontiers in microbiology · 2022Review
- Diagnosis ofInfection and drug resistance · 2022Article
- Next-Generation Sequencing Technology Combined With Multiplex Polymerase Chain Reaction as a Powerful Detection and Semiquantitative Method for Herpes Simplex Virus Type 1 in Adult Encephalitis: A Case Report.Frontiers in medicine · 2022Article
- Metagenomic next-generation sequencing for identification of central nervous system pathogens in HIV-infected patients.Frontiers in microbiology · 2022Article
Corrections and comments
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Authors and funding
7 authors.
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Abstract
backgroundMore than 100 different pathogens can cause encephalitis. Testing of all the neurological pathogens by conventional methods can be difficult. Metagenomic next-generation sequencing (NGS) could identify the infectious agents in a target-independent manner. The role of this novel method in clinical diagnostic microbiology still needs to be evaluated. In present study, we used metagenomic NGS to search for an infectious etiology in a human immunodeficiency virus (HIV)-infected patient with lethally diffuse brain lesions. Sequences mapping to Toxoplasma gondii were unexpectedly detected. CASE PRESENTATION: A 31-year-old HIV-infected patient presented to hospital in a critical ill condition with a Glasgow coma scale score of 3. Brain magnetic resonance imaging showed diffuse brain abnormalities with contrast enhancement. Metagenomic NGS was performed on DNA extract from 300 μL patient's cerebrospinal fluid (CSF) with the BGISEQ-50 platform. The sequencing detection identified 65,357 sequence reads uniquely aligned to the Toxoplasma gondii genome. Presence of Toxoplasma gondii genome in CSF was further verified by Toxoplasma gondii-specific polymerase chain reaction and Sanger sequencing. Altogether, those results confirmed the diagnosis of toxoplasmic encephalitis.
conclusionsThis study suggests that metagenomic NGS may be a useful diagnostic tool for toxoplasmic encephalitis. As metagenomic NGS is able to identify all pathogens in a single run, it may be a promising strategy to explore the clinical causative pathogens in central nervous system infections with atypical features.
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