ArticleThe Histochemical journal1988
LR-White and LR-Gold resins for postembedding immunofluorescence staining of laminin in mouse kidney.
Article in The Histochemical journal, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed, 9 citations in OpenAlex.
- Array tomography: a new tool for imaging the molecular architecture and ultrastructure of neural circuits.Neuron · 2007Article
- Postembedding immunogold histochemistry for the localization of laminin and its E4 and P1 fragments in mouse kidney embedded in LR-White and LR-Gold.The Histochemical journal · 1991Article
- Appearance of laminin, heparan sulphate proteoglycan and collagen type IV during initial stages of vascularisation of the neuroepithelium of the mouse embryo.Journal of anatomy · 1990Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This work describes a method for the immunolocalization of laminin on 1 micron-thick tissue sections using a postembedding immunofluorescence technique. Embedding of unfixed or formaldehyde-fixed mouse renal cortex in either of the acrylic resins LR-White or LR-Gold permitted reliable postembedding immunofluorescence staining for laminin. LR-White was heat-cured at 50 degrees C whereas LR-Gold was polymerized at -25 degrees C. A stronger immunostaining for laminin was obtained from tissue embedded in polymerized LR-Gold compared with the staining from tissue embedded in LR-White. Prerequisites for adequate postembedding immunostaining are the partial dehydration of the tissue (maximum ethanol concentration, 70%) and pepsin treatment of the tissue sections prior to performing the immunostaining reactions.
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Registered trials
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