ArticleBiochemistry2019
A Robust Method for the Purification and Characterization of Recombinant Human Histone H1 Variants.
Article in Biochemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Article
- Histone variant H2BE enhances chromatin accessibility in neurons to promote synaptic gene expression and long-term memory.Molecular cell · 2024Article
- A nucleosome switch primes Hepatitis B Virus infection.bioRxiv : the preprint server for biology · 2024Article
- Histone variant H2BE enhances chromatin accessibility in neurons to promote synaptic gene expression and long-term memory.bioRxiv : the preprint server for biology · 2024Article
- Simplified cloning and isolation of peptides from "sandwiched" SUMO-peptide-intein fusion proteins.BMC biotechnology · 2023Article
- Chromatin sequesters pioneer transcription factor Sox2 from exerting force on DNA.Nature communications · 2022Article
- 3D chromosomal architecture in germinal center B cells and its alterations in lymphomagenesis.Current opinion in genetics & development · 2022Review
- Histone H1 binding to nucleosome arrays depends on linker DNA length and trajectory.Nature structural & molecular biology · 2022Article
- Single-stranded nucleic acid binding and coacervation by linker histone H1.Nature structural & molecular biology · 2022Article
- SPI "sandwich": Combined SUMO-Peptide-Intein expression system and isolation procedure for improved stability and yield of peptides.Protein science : a publication of the Protein Society · 2022Article
- SUMOylation of linker histone H1 drives chromatin condensation and restriction of embryonic cell fate identity.Molecular cell · 2022Article
- Histone H1 Mutations in Lymphoma: A Link(er) between Chromatin Organization, Developmental Reprogramming, and Cancer.Cancer research · 2021Review
- Site-specific ubiquitylation acts as a regulator of linker histone H1.Nature communications · 2021Article
- Targeting Hepatitis B Virus Covalently Closed Circular DNA and Hepatitis B Virus X Protein: Recent Advances and New Approaches.ACS infectious diseases · 2019Article
- (De)Toxifying the Epigenetic Code.Chemical research in toxicology · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Higher order compaction of the eukaryotic genome is key to the regulation of all DNA-templated processes, including transcription. This tightly controlled process involves the formation of mononucleosomes, the fundamental unit of chromatin, packaged into higher order architectures in an H1 linker histone-dependent process. While much work has been done to delineate the precise mechanism of this event in vitro and in vivo, major gaps still exist, primarily due to a lack of molecular tools. Specifically, there has never been a successful purification and biochemical characterization of all human H1 variants. Here we present a robust method to purify H1 and illustrate its utility in the purification of all somatic variants and one germline variant. In addition, we performed a first ever side-by-side biochemical comparison, which revealed a gradient of nucleosome binding affinities and compaction capabilities. These data provide new insight into H1 redundancy and lay the groundwork for the mechanistic investigation of disease-driving mutations.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.