ArticleThe New phytologist2019
Multilab EcoFAB study shows highly reproducible physiology and depletion of soil metabolites by a model grass.
Article in The New phytologist, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 86 citations in OpenAlex.
- Systems-Level Plant Responses Reveal Pseudomonas-Mediated Growth Promotion in Brachypodium Under Nitrogen Limitation.Plant, cell & environment · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Synthetic soils for ecological and synthetic biology applications.FEMS microbiology reviews · 2026Review
- Synchronizing Germination Rates Across Plant Species for Fabricated Ecosystems EcoFAB 2.0.Bio-protocol · 2025Article
- Bacterial fitness for plant colonization is influenced by plant growth substrate.The New phytologist · 2025Article
- Label-free structural imaging of plant roots and microbes using third-harmonic generation microscopy.Scientific reports · 2025Article
- The chemical interaction between plants and the rhizosphere microbiome.Trends in plant science · 2025Review
- Breaking the reproducibility barrier with standardized protocols for plant-microbiome research.PLoS biology · 2025Article
- Assessing horizontal gene transfer in the rhizosphere ofApplied and environmental microbiology · 2024Article
- RhizoNet segments plant roots to assess biomass and growth for enabling self-driving labs.Scientific reports · 2024Article
- Where bacteria and eukaryotes meet.Journal of bacteriology · 2024Article
- Emerging sensing, imaging, and computational technologies to scale nano-to macroscale rhizosphere dynamics - Review and research perspectives.Soil biology & biochemistry · 2024Article
- Reproducible growth ofScience advances · 2024Article
- Fabricated devices for performing bacterial-fungal interaction experiments across scales.Frontiers in microbiology · 2024Review
- Arabidopsis Root Microbiome Microfluidic (ARMM) Device for Imaging Bacterial Colonization and Morphogenesis of Arabidopsis Roots.Methods in molecular biology (Clifton, N.J.) · 2024Article
- EcoFAB 3.0: a sterile system for studying sorghum that replicates previous field and greenhouse observations.Frontiers in plant science · 2024Article
- Studying root-environment interactions in structured microdevices.Journal of experimental botany · 2023Article
- Fine scale sampling reveals early differentiation of rhizosphere microbiome from bulk soil in young Brachypodium plant roots.ISME communications · 2023Article
- A Reproducible and Tunable Synthetic Soil Microbial Community Provides New Insights into Microbial Ecology.mSystems · 2022Article
- The Promises, Challenges, and Opportunities of Omics for Studying the Plant Holobiont.Microorganisms · 2022Article
Corrections and comments
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Authors and funding
17 authors at 4 institutions in 3 countries.
Funding
Abstract
There is a dynamic reciprocity between plants and their environment: soil physiochemical properties influence plant morphology and metabolism, and root morphology and exudates shape the environment surrounding roots. Here, we investigate the reproducibility of plant trait changes in response to three growth environments. We utilized fabricated ecosystem (EcoFAB) devices to grow the model grass Brachypodium distachyon in three distinct media across four laboratories: phosphate-sufficient and -deficient mineral media allowed assessment of the effects of phosphate starvation, and a complex, sterile soil extract represented a more natural environment with yet uncharacterized effects on plant growth and metabolism. Tissue weight and phosphate content, total root length, and root tissue and exudate metabolic profiles were consistent across laboratories and distinct between experimental treatments. Plants grown in soil extract were morphologically and metabolically distinct, with root hairs four times longer than with other growth conditions. Further, plants depleted half of the metabolites investigated from the soil extract. To interact with their environment, plants not only adapt morphology and release complex metabolite mixtures, but also selectively deplete a range of soil-derived metabolites. The EcoFABs utilized here generated high interlaboratory reproducibility, demonstrating their value in standardized investigations of plant traits.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.