ArticleParasites & vectors2018
Isolation and characterization of a temperature-sensitive lethal strain of Anopheles arabiensis for SIT-based application.
Article in Parasites & vectors, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 16 citations in OpenAlex.
- Synthetic biology approaches to generate temperature-sensitive alleles for the Sterile Insect Technique.Insect science · 2026Review
- Selection, counterselection, and conditional gene expression for genetic biocontrol of insects.Insect science · 2026Review
- Decoding and engineering temperature-sensitive lethality inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Trends in insecticide resistance in natural populations of Culex quinquefasciatus and its impact on mosquito fitness in Dschang, West Cameroon.Parasitology research · 2025Article
- Article
- An update on the mosquito fauna and mosquito-borne diseases distribution in Cameroon.Parasites & vectors · 2021Review
- Review
- Joint FAO/IAEA Coordinated Research Project on "Exploring genetic, molecular, mechanical and behavioural methods of sex separation in mosquitoes" - an introduction.Parasites & vectors · 2018Article
- A review on the progress of sex-separation techniques for sterile insect technique applications against Anopheles arabiensis.Parasites & vectors · 2018Review
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMalaria is still a global health problem and vector control is the cornerstone of disease control strategies using indoor residual insecticide spraying (IRS) and insecticide-treated nets. The situation is becoming acute with widespread resistance to the limited arsenal of available insecticide classes. Therefore, new and innovative tools to reduce Plasmodium transmission are in need and this situation raised considerable interest in using sterile insect technique (SIT) against human pest insects, particularly Anopheles malaria vectors. When considering a mosquito release programme, one of the first issues to be addressed is how to eliminate/separate the hematophagous vector females. In this paper, we report the development and evaluation of an Anopheles arabiensis temperature-sensitive lethal (tsl) strain towards its potential use for the development of a genetic sexing strain (GSS). Anopheles arabiensis male mosquitoes originated from North Cameroon were treated with 0.05% ethyl methanesulfonate (EMS). The mutagen was added to 10% sucrose solution and mosquitoes fed ad-libitum for 24h and 48h on a cotton wool soaked with sucrose-mutagen solution placed at the top of the cup. Treated males were then allowed to mate with wild virgin females and their progenies were screened for heat sensitivity from F3 to F8, until the isolation and establishment of a tsl strain which was further characterized by assessing its productivity (fecundity and fertility), larval development, adult longevity as well as nature and inheritance pattern of the tsl.
resultsObservations showed that the number of eggs laid and their hatch rate were similar between females that mated with mutagenized males and those from the control suggesting that mutagenesis didn't affect An. arabiensis male fecundity and fertility. At F3, fourteen isofemale families out of 88 tested, which showed mortalities ranged between 50 and 80 %, were selected as lines potentially containing a tsl mutation. From F4 to F8, a tsl strain was isolated and established by screening L1 larvae at 41°C for 3 hours. This strain showed similar life history traits compared to the wild type strain in terms of fertility, larval development time and adult's emergence. Crossing experiments to further assess the nature and inheritance pattern of the tsl phenotype showed that it is due to a recessive allele located on an autosome.
conclusionThe successful establishment of the An. arabiensis tsl strain is a valuable tool towards the development of a GSS for SIT applications against this species. This will be done by induction of a Y-autosome translocation to link the wild-type allele to the Y chromosome in such manner that males are wild-type (temperature resistant) and females are mutant (temperature sensitive).
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