ArticleMolecular microbiology2019
Contributions of crust proteins to spore surface properties in Bacillus subtilis.
Article in Molecular microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 65 citations in OpenAlex.
- Distinctive spore architecture and developmental biology ofJournal of bacteriology · 2026Article
- Expression of theApplied and environmental microbiology · 2026Article
- Identification of a core set ofMicrobiology (Reading, England) · 2026Article
- Spore immobilized enzymes for the multi-step synthesis of cellobiose.Microbial cell factories · 2026Article
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- Bacillus subtilis as cell factory for enhanced production of the biopolymer precursor pyridine-2,6-dicarboxylic acid.Microbial cell factories · 2025Article
- Genomic analysis of Arthrobacter endolithicus sp. nov. and Arthrobacter antibioticus sp. nov., two novel actinobacteria from Antarctica with potential application in aquaculture.Antonie van Leeuwenhoek · 2025Article
- Effects of extreme acceleration, microgravity, and deceleration on Bacillus subtilis onboard a suborbital space flight.NPJ microgravity · 2025Article
- Cryo-EM structure of the glycosylated protein CgeA in the crust of Bacillus subtilis endospores.Journal of microbiology (Seoul, Korea) · 2025Article
- Conservation of sporulation genes and a transmembrane-containing Spo0B variant inbioRxiv : the preprint server for biology · 2025Article
- Immunostimulatory activity of inactivated environmental Bacillus isolates and their endospores.Scientific reports · 2025Article
- Real-time nanoscale investigation of spore coat assembly in Bacillus subtilis.Communications biology · 2025Article
- Sporulation at reduced water activity impairs germination kinetics ofApplied and environmental microbiology · 2025Article
- Carrier immobilization and auto-exposition favoring reuse of silyletherase SilE-R from Brassica sp. with high activity and enantiospecificity.Biotechnology letters · 2025Article
- Disruption of Spore Coat Integrity inCurrent issues in molecular biology · 2025Article
- High Genetic Diversity AmongMicroorganisms · 2025Article
- Immobilization of alginate C-5 epimerases usingApplied and environmental microbiology · 2025Article
- Embedding a ribonuclease in the spore crust couples gene expression to spore development in Bacillus subtilis.Nucleic acids research · 2025Article
- Article
- Combination of fluorescent reagents with 2-(4-aminophenyl) benzothiazole and safranin O was useful for analysis of spore structure, indicating the diversity ofFrontiers in microbiology · 2025Article
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
Surface properties, such as adhesion and hydrophobicity, constrain dispersal of bacterial spores in the environment. In Bacillus subtilis, these properties are influenced by the outermost layer of the spore, the crust. Previous work has shown that two clusters, cotVWXYZ and cgeAB, encode the protein components of the crust. Here, we characterize the respective roles of these genes in surface properties using Bacterial Adherence to Hydrocarbons assays, negative staining of polysaccharides by India ink and Transmission Electron Microscopy. We showed that inactivation of crust genes caused increases in spore relative hydrophobicity, disrupted the spore polysaccharide layer, and impaired crust structure and attachment to the rest of the coat. We also found that cotO, previously identified for its role in outer coat formation, is necessary for proper encasement of the spore by the crust. In parallel, we conducted fluorescence microscopy experiments to determine the full network of genetic dependencies for subcellular localization of crust proteins. We determined that CotZ is required for the localization of most crust proteins, while CgeA is at the bottom of the genetic interaction hierarchy.
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