Frequency difference mapping applied to the corpus callosum at 7T.

purposeFrequency difference mapping (FDM) is a phase processing technique which characterizes the nonlinear temporal evolution of the phase of gradient echo (GE) signals. Here, a novel FDM-processing algorithm is introduced, which is shown to reveal information about white matter microstructure. Unlike some other phase-processing techniques, the FDM algorithm presented here does not require the use of phase unwrapping or sophisticated image processing. It uses a series of scaled complex divisions to unwrap phase and remove background fields.

methodsTen healthy subjects underwent a series of single-slice, sagittal multi-echo GE scans at 7T with the slice positioned at the midline. Phase data were processed with the novel FDM algorithm, and the temporal evolution of the magnitude signal and frequency difference was examined in 5 regions of the corpus callosum (CC; genu, anterior body, middle body, posterior body, and splenium).

resultsConsistent frequency difference contrast relative to surrounding tissue was observed in all subjects in the CC and in other white matter regions where the nerve fibers run perpendicular to

conclusionThe novel FDM algorithm presented here yields images sensitive to tissue microstructure and microstructural differences over the CC in a simple manner, without the requirement for phase unwrapping or sophisticated image processing.