ArticleThe Laryngoscope2019
Subacute acrolein exposure to rat larynx in vivo.
Article in The Laryngoscope, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 7 citations in OpenAlex.
- Socioeconomic Deprivation and Vocal Handicap in Adults With Voice Disorders.The Laryngoscope · 2026Article
- Socioeconomic Status, Voice Disorder Risk, and Voice-Related Handicap Across Childhood.JAMA otolaryngology-- head & neck surgery · 2025Article
- Furosemide-induced systemic dehydration alters the proteome of rabbit vocal folds.Journal of proteomics · 2022Article
- Lung injury caused by exposure to the gaseous fraction of exhaust from biomass combustion (cashew nut shells): a mice model.Environmental science and pollution research international · 2020Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
objectives/hypothesisInhaled pollutants can contact vocal fold tissue and induce detrimental voice changes. Acrolein is a pollutant in cigarette smoke and can also be inhaled during the combustion of fossil fuels, animal fats, and plastics in the environment. However, the vocal fold pathological changes induced by acrolein and the underlying inflammatory pathways are not well understood. These biologic data are needed to understand why voice problems may result from pollutant exposure. STUDY
designIn vivo prospective design with experimental and control groups.
methodsSprague-Dawley male rats (N = 36) were exposed to acrolein (3 ppm) or filtered air (control) through a whole-body exposure system for 5 hours/day, for 5 days/week, over 4 weeks. Histopathological changes, presence of edema, expression of proinflammatory cytokines and markers, and the phosphorylation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) were investigated.
resultsHistological evaluation and quantification demonstrated that subacute acrolein exposure induced significant vocal fold edema. Acrolein exposure also induced epithelial sloughing and cell death. Quantitative polymerase chain reaction showed a significant upregulation of genes encoding interferon regulatory factor and chitinase-3-like protein 3. Western blot revealed a 76.8% increase in phosphorylation of NF-κB P65 after subacute acrolein exposure.
conclusionsThese findings suggest that 4-week exposures to 3 ppm acrolein induce vocal fold inflammation manifested as edema, related to the activation of NF-κB signaling. The edema may underlie the voice changes reported in speakers exposed to pollutants. LEVEL OF EVIDENCE: NA Laryngoscope, 129:E313-E317, 2019.
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Registered trials
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