ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2018
Label-Free High-Throughput Leukemia Detection by Holographic Microscopy.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 79 citations in OpenAlex.
- Label-free single-cell phenotyping to determine tumor cell heterogeneity in pancreatic cancer in real time.JCI insight · 2025Article
- From genotype to phenotype: decoding mutations in blasts by holo-tomographic flow cytometry.Light, science & applications · 2025Article
- Spatial microfluidic holographic integrated platform for label-free and high-dimensional analysis of cancer heterogeneity.Nature communications · 2025Article
- OAH-Net: a deep neural network for efficient and robust hologram reconstruction for off-axis digital holographic microscopy.Biomedical optics express · 2025Article
- Characterizing the particulate content of urine in healthy humans using flow cytometry.PloS one · 2025Article
- Rapid mechanical phenotyping of breast cancer cells based on stochastic intracellular fluctuations.iScience · 2024Article
- Digital Holographic Microscopy in Veterinary Medicine-A Feasibility Study to Analyze Label-Free Leukocytes in Blood and Milk of Dairy Cows.Animals : an open access journal from MDPI · 2024Article
- Off-axis digital lensless holographic microscopy based on spatially multiplexed interferometry.Journal of biomedical optics · 2024Article
- Analyzing Blood Cells of High-Risk Myelodysplastic Syndrome Patients Using Interferometric Phase Microscopy and Fluorescent Flow Cytometry.Bioengineering (Basel, Switzerland) · 2024Article
- Platelet aggregates detected using quantitative phase imaging associate with COVID-19 severity.Communications medicine · 2023Article
- Phenotyping neuroblastoma cells through intelligent scrutiny of stain-free biomarkers in holographic flow cytometry.APL bioengineering · 2023Article
- Article
- Deep learning assisted holography microscopy for in-flow enumeration of tumor cells in blood.RSC advances · 2023Article
- Label-free hematology analysis method based on defocusing phase-contrast imaging under illumination of 415 nm light.Biomedical optics express · 2022Article
- Single-Shot 3D Topography of Transmissive and Reflective Samples with a Dual-Mode Telecentric-Based Digital Holographic Microscope.Sensors (Basel, Switzerland) · 2022Article
- Observational
- Article
- Rapid, label-free classification of tumor-reactive T cell killing with quantitative phase microscopy and machine learning.Scientific reports · 2021Article
- High-speed laser-scanning biological microscopy using FACED.Nature protocols · 2021Article
- Deepometry, a framework for applying supervised and weakly supervised deep learning to imaging cytometry.Nature protocols · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Complete blood count and differentiation of leukocytes (DIFF) belong to the most frequently performed laboratory diagnostic tests. Here, a flow cytometry-based method for label-free DIFF of untouched leukocytes by digital holographic microscopy on the rich phase contrast of peripheral leukocyte images, using highly controlled 2D hydrodynamic focusing conditions is reported. Principal component analysis of morphological characteristics of the reconstructed images allows classification of nine leukocyte types, in addition to different types of leukemia and demonstrates disappearance of acute myeloid leukemia cells in remission. To exclude confounding effects, the classification strategy is tested by the analysis of 20 blinded clinical samples. Here, 70% of the specimens are correctly classified with further 20% classifications close to a correct diagnosis. Taken together, the findings indicate a broad clinical applicability of the cytometry method for automated and reagent-free diagnosis of hematological disorders.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.