ArticleFrontiers in microbiology2018
Environmental DNA: A New Low-Cost Monitoring Tool for Pathogens in Salmonid Aquaculture.
Article in Frontiers in microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Research on Quantitative Analysis Technology for Pacific White Shrimp Density Based on Environmental DNA.Ecology and evolution · 2026Article
- Environmental DNA screening of Phocoenobacter atlanticus subsp. atlanticus in Atlantic salmon aquaculture.PloS one · 2026Article
- The Genomics Revolution in Nonmodel Species: Predictions vs. Reality for Salmonids.Molecular ecology · 2025Review
- Submissions of diagnostic samples of two critically endangered species of handfish (Branchionichthys hirsutus and Thymichthys politus) from a public aquarium from 2018 to 2024.Journal of fish diseases · 2025Article
- Synthetic spike-in metabarcoding for plant pathogen diagnostics results in precise quantification of copy number within the genusISME communications · 2025Article
- Application of an eDNA assay for the detection ofAquaculture (Amsterdam, Netherlands) · 2023Article
- Article
- MULTI-STATE OCCUPANCY MODEL ESTIMATES PROBABILITY OF DETECTION OF AN AQUATIC PARASITE USING ENVIRONMENTAL DNA: PSEUDOLOMA NEUROPHILIA IN ZEBRAFISH AQUARIA.The Journal of parasitology · 2022Article
- Recent advances and public health implications for environmental exposure to Chlamydia abortus: from enzootic to zoonotic disease.Veterinary research · 2022Review
- Honey Environmental DNA Can Be Used to Detect and Monitor Honey Bee Pests: Development of Methods Useful to IdentifyVeterinary sciences · 2022Article
- Gut microbiota diversity in human strongyloidiasis differs little in two different regions in endemic areas of Thailand.PloS one · 2022Article
- An Efficient Tetraplex Surveillance Tool for Salmonid Pathogens.Frontiers in microbiology · 2022Article
- Sea lice (Lepeophtherius salmonis) detection and quantification around aquaculture installations using environmental DNA.PloS one · 2022Article
- Human Fecal Contamination Corresponds to Changes in the Freshwater Bacterial Communities of a Large River Basin.Microbiology spectrum · 2021Article
- Illumina iSeq 100 and MiSeq exhibit similar performance in freshwater fish environmental DNA metabarcoding.Scientific reports · 2021Article
- Water pre-filtration methods to improve environmental DNA detection by real-time PCR and metabarcoding.PloS one · 2021Article
- Application of next generation sequencing for detection of protozoan pathogens in shellfish.Food and waterborne parasitology · 2020Article
- Review of diseases and health management in zebrafish Danio rerio (Hamilton 1822) in research facilities.Journal of fish diseases · 2020Review
- Everything is not everywhere: can marine compartments shape phytoplankton assemblages?Proceedings. Biological sciences · 2019Article
- Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Environmental DNA (eDNA) metabarcoding is a relatively new monitoring tool featuring in an increasing number of applications such as the facilitation of the accurate and cost effective detection of species in environmental samples. eDNA monitoring is likely to have a major impact on the ability of salmonid aquaculture industry producers and their regulators to detect the presence and abundance of pathogens and other biological threats in the surrounding environment. However, for eDNA metabarcoding to develop into a useful bio-monitoring tool it is necessary to (a) validate that sequence datasets derived from amplification of metabarcoding markers reflect the true species' identity, (b) test the sensitivity under different abundance levels and environmental noise and (c) establish a low-cost sequencing method to enable the bulk processing of field samples. In this study, we employed an elaborate experimental design whereby different combinations of five biological agents were crossed at three abundance levels and exposed to sterile pre-filtered and unfiltered seawater, prior to coarse filtering and then eDNA ultrafiltration of the resultant material. We then benchmarked the low-cost, scalable, Ion Torrent sequencing method against the current gold-standard Illumina platform for eDNA surveys in aquaculture. Based on amplicon-seq of the 18S SSU rDNA v9 region, we were able to identify two parasites (
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