ArticleMolecular metabolism2019
A dual Ucp1 reporter mouse model for imaging and quantitation of brown and brite fat recruitment.
Article in Molecular metabolism, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 48 citations in OpenAlex.
- Brown Adipose Tissue in Breast Cancer Evaluated by [Molecular imaging and biology · 2020Trial
- A UCP1-IRES-Cre Knock-In Mouse Enables Specific Brown Adipocyte Targeting Without CNS Off-Target Expression.Research square · 2026Article
- Illuminating the metabolic sink: Optical probes for in vivo thermogenic fat imaging.Materials today. Bio · 2026Review
- NCOA1 is a gatekeeper of the sexually dimorphic thermogenic activity of white adipose tissue.Nature communications · 2026Article
- Salt-inducible Kinase Regulation of Adipose Tissue Metabolism.Endocrinology · 2025Review
- No UCP1 in the kidney.Molecular metabolism · 2025Article
- The widely used Ucp1-Cre transgene elicits complex developmental and metabolic phenotypes.Nature communications · 2025Article
- Amphiphilic cytokine traps remodel marrow adipose tissue for hematopoietic microenvironment amelioration.Bioactive materials · 2024Article
- Gene expression and characterization of clonally derived murine embryonic brown and brite adipocytes.FEBS open bio · 2024Article
- Development and expansion of intramuscular adipose tissue is not dependent on UCP-1-lineage cells in mice.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2023Article
- Sensing the oxygen and temperature in the adipose tissues - who's sensing what?Experimental & molecular medicine · 2023Review
- Uncoupling protein 1-driven Cre (bioRxiv : the preprint server for biology · 2023Article
- The widely usedbioRxiv : the preprint server for biology · 2023Article
- Use of CIDEA Reporter Mouse Model for Screening Thermogenic Fat-Activating Drugs.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Human Umbilical Vein Endothelial Cells Survive on the Ischemic TCA Cycle under Lethal Ischemic Conditions.Journal of proteome research · 2022Article
- Visualization and quantification ofAnnals of translational medicine · 2022Article
- Loss of UCP1 function augments recruitment of futile lipid cycling for thermogenesis in murine brown fat.Molecular metabolism · 2022Article
- Application and trend of bioluminescence imaging in metabolic syndrome research.Frontiers in chemistry · 2022Review
- Multi-Aspect Optoacoustic Imaging of Breast Tumors under Chemotherapy with Exogenous and Endogenous Contrasts: Focus on Apoptosis and Hypoxia.Biomedicines · 2021Article
- Mouse model of the adipose organ: the heterogeneous anatomical characteristics.Archives of pharmacal research · 2021Review
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesBrown adipose tissue (BAT) dissipates nutritional energy as heat through uncoupling protein 1 (UCP1). The discovery of functional BAT in healthy adult humans has promoted the search for pharmacological interventions to recruit and activate brown fat as a treatment of obesity and diabetes type II. These efforts require in vivo models to compare the efficacy of novel compounds in a relevant physiological context.
methodsWe generated a knock-in mouse line expressing firefly luciferase and near-infrared red florescent protein (iRFP713) driven by the regulatory elements of the endogenous Ucp1 gene.
resultsOur detailed characterization revealed that firefly luciferase activity faithfully reports endogenous Ucp1 gene expression in response to physiological and pharmacological stimuli. The iRFP713 fluorescence signal was detected in the interscapular BAT region of cold-exposed reporter mice in an allele-dosage dependent manner. Using this reporter mouse model, we detected a higher browning capacity in female peri-ovarian white adipose tissue compared to male epididymal WAT, which we further corroborated by molecular and morphological features. In situ imaging detected a strong luciferase activity signal in a previously unappreciated adipose tissue depot adjunct to the femoral muscle, now adopted as femoral brown adipose tissue. In addition, screening cultured adipocytes by bioluminescence imaging identified the selective Salt-Inducible Kinase inhibitor, HG-9-91-01, to increase Ucp1 gene expression and mitochondrial respiration in brown and brite adipocytes.
conclusionsIn our mouse model, firefly luciferase activity serves as a bona fide reporter for dynamic regulation of Ucp1. In addition, by means of iRFP713 we are able to monitor Ucp1 expression in a non-invasive fashion.
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