Evidence map›Paper›PMID 30580661›Full record

ArticleFly2018

A model-based high throughput method for fecundity estimation in fruit fly studies.

Enoch Ng'oma, Elizabeth G King, Kevin M Middleton

Open access · bronzeAbstract read
In one paragraph

Article in Fly, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.6field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. A high-throughput method for quantifyingbioRxiv : the preprint server for biology · 2024
    Article
  6. Phenotyping ofBiomolecules · 2022
    Review
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Enoch Ng'omaa Division of Biological Sciences , University of Missouri , Columbia , MO , USA.ORCID 0000-0002-6741-7922
Elizabeth G Kinga Division of Biological Sciences , University of Missouri , Columbia , MO , USA.ORCID 0000-0002-9393-4720
Kevin M Middletonb Department of Pathology and Anatomical Sciences , University of Missouri School of Medicine , Columbia , MO , USA.ORCID 0000-0003-4704-1064
University of Missouri · US

Funding

Coordinating nutrition and energy allocation: mechanisms and evolutionR01GM117135 · NIGMS · UNIVERSITY OF MISSOURI-COLUMBIA · PI KING, ELIZABETH GRIEP · 2016 to 2020
$1.6M
NIGMS NIH HHS R01 GM117135
6 · The paper itself

Abstract

The ability to quantify fecundity is critically important to a wide range of experimental applications, particularly in widely-used model organisms such as Drosophila melanogaster. However, the standard method of manually counting eggs is time consuming and limits the feasibility of large-scale experiments. We develop a predictive model to automate the counting of eggs from images of eggs removed from the media surface and washed onto dark filter paper. Our method uses the simple relationship between the white area in an image and the number of eggs present to create a predictive model that performs well even at high egg densities where clumping can complicate the individual identification of eggs. A cross-validation approach demonstrates our method performs well, with a correlation between predicted and manually counted values of 0.88. We show how this method can be applied to a large data set where egg densities vary widely.

Indexed as

AnimalsDrosophila melanogasterFemaleFertilityHigh-Throughput Screening AssaysImage Processing, Computer-AssistedMaleOvipositionOvumcross-validationfecundityImage analysisquantitative biological imaging

Identifiers

PMID30580661
PMCPMC6988892
OpenAlexW2949923802

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.