ArticleAnalytical and bioanalytical chemistry2019
A method for the quantitative determination of glycerophospholipid regioisomers by UPLC-ESI-MS/MS.
Article in Analytical and bioanalytical chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 24 citations in OpenAlex.
- New Analytical Technologies to Resolve, Interpret, and Understand Lipid Complexity.Annual review of analytical chemistry (Palo Alto, Calif.) · 2026Review
- Isomer Composition Assessment of Synthetic Phosphatidylethanol by Collision-Induced and Ozone-Induced Dissociation Mass Spectrometry.Drug testing and analysis · 2026Article
- prm-PASEF-Based Quantification and Isomeric Model for Extended Coverage of Human Plasma Lipidome in Parkinson's Disease.Analytical chemistry · 2025Article
- Spatially and temporally probing distinctive glycerophospholipid alterations in Alzheimer's disease mouse brain via high-resolution ion mobility-enabled sn-position resolved lipidomics.Nature communications · 2024Article
- Recent Analytical Methodologies in Lipid Analysis.International journal of molecular sciences · 2024Review
- Isomeric lipid signatures reveal compartmentalized fatty acid metabolism in cancer.Journal of lipid research · 2022Article
- Relative Quantitation of Unsaturated Phosphatidylcholines Using 193 nm Ultraviolet Photodissociation Parallel Reaction Monitoring Mass Spectrometry.Journal of the American Chemical Society · 2021Article
- Recommendations for good practice in MS-based lipidomics.Journal of lipid research · 2021Review
- Mapping Unsaturation in Human Plasma Lipids by Data-Independent Ozone-Induced Dissociation.Journal of the American Society for Mass Spectrometry · 2019Article
- Accurate mass and retention time library of serum lipids for type 1 diabetes research.Analytical and bioanalytical chemistry · 2019Article
- Analysis of triacylglycerol and phospholipid sn-positional isomers by liquid chromatographic and mass spectrometric methodologies.Mass spectrometry reviewsReview
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
Diacyl glycerophospholipids (GPs) belong to the most abundant lipid species in living organisms and consist of a glycerol backbone with fatty acyl groups in sn-1 and sn-2 and a polar head group in the sn-3 position. Regioisomeric mixed diacyl GPs have the same fatty acyl composition but differ in their allocation to sn-1 or sn-2 of the glycerol unit. In-depth analysis of regioisomeric mixed diacyl GP species composed of fatty acyl moieties that are similar in length and degree of saturation typically requires either chemical derivatization or sophisticated analytical instrumentation, since these types of regioisomers are not well resolved under standard ultra-performance liquid chromatography (UPLC) conditions. Here, we introduce a simple and fast method for diacyl GP regioisomer analysis employing UPLC tandem mass spectrometry (MS/MS). This GP regioisomer analysis is based both on minor chromatographic retention time shifts and on major differences in relative abundances of the two fatty acyl anion fragments observed in MS/MS. To monitor these differences with optimal precision, MS/MS spectra are recorded continuously over the UPLC elution profile of the lipid species of interest. Quantification of relative abundances of the regioisomers was performed by algorithms that we have developed for this purpose. The method was applied to commercially available mixed diacyl GP standards and to total lipid extracts of Escherichia coli (E. coli) and bovine liver. To validate our results, we determined regioisomeric ratios of phosphatidylcholine (PC) standards using phospholipase A
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