ArticleJournal of biomedical optics2018
Detection of glycosaminoglycan loss in articular cartilage by fluorescence lifetime imaging.
Article in Journal of biomedical optics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 37 citations in OpenAlex.
- Distinct adhesion energy and extracellular matrix profiles of healthy and osteoarthritic human chondrocytes.In vitro cellular & developmental biology. Animal · 2026Article
- Evaluation of Partial Volume Correction Techniques for Sodium MRI of the Achilles Tendon.Magnetic resonance in medicine · 2026Article
- Assessing the micromechanical architecture of joint tissues with speckle rheological microscopy.Nature communications · 2026Article
- Evaluation of Engineered Cartilage Composition and Function Using Raman Spectroscopy.Advanced healthcare materials · 2025Article
- Beyond the Surface: Nutritional Interventions Integrated with Diagnostic Imaging Tools to Target and Preserve Cartilage Integrity: A Narrative Review.Biomedicines · 2025Review
- In Vitro Analysis of Human Cartilage Infiltrated by Hydrogels and Hydrogel-Encapsulated Chondrocytes.Bioengineering (Basel, Switzerland) · 2023Article
- Pulsed Electromagnetic Field Therapy and Direct Current Electric Field Modulation Promote the Migration of Fibroblast-like Synoviocytes to Accelerate Cartilage Repair In Vitro.Applied sciences (Basel, Switzerland) · 2022Article
- Non-destructive, continuous monitoring of biochemical, mechanical, and structural maturation in engineered tissue.Scientific reports · 2022Article
- Changes in T2 Relaxation Time Mapping of Intervertebral Discs Adjacent to Vertebrae after Kyphoplasty Correlate with the Physical Clinical Outcome of Patients.Diagnostics (Basel, Switzerland) · 2022Article
- Nondestructive testing of native and tissue-engineered medical products: adding numbers to pictures.Trends in biotechnology · 2022Review
- Multispectral fluorescence lifetime imaging device with a silicon avalanche photodetector.Optics express · 2021Article
- Mesoscopic fluorescence lifetime imaging: Fundamental principles, clinical applications and future directions.Journal of biophotonics · 2021Review
- Real-time multispectral fluorescence lifetime imaging using Single Photon Avalanche Diode arrays.Scientific reports · 2020Article
- Autofluorescence Lifetime Reports Cartilage Damage in Osteoarthritis.Scientific reports · 2020Article
- Optical fiber-based dispersion for spectral discrimination in fluorescence lifetime imaging systems.Journal of biomedical optics · 2019Article
- Non-destructive detection of matrix stabilization correlates with enhanced mechanical properties of self-assembled articular cartilage.Journal of tissue engineering and regenerative medicine · 2019Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Glycosaminoglycan (GAG) loss is an early marker of osteoarthritis, which is a clinical late stage disease that affects millions of people worldwide. The goal of our study was to evaluate the ability of a fiber-based fluorescence lifetime imaging (FLIm) technique to detect GAG loss in articular cartilage. Native bovine cartilage explants (n = 20) were exposed to 0 (control), 0.5 (low), or 1 U / mL (high) concentrations of chondroitinase ABC (cABC) to create samples with different levels of GAG loss. FLIm assessment (excitation: 355 nm; detection: channel 1: 375 to 410 nm, channel 2: 450 to 485 nm, channel 3: 530 to 565 nm) was conducted on depth-resolved cross-sections of the cartilage sample. FLIm images, validated with histology, revealed that loss of GAG resulted in a decrease of fluorescence lifetime values in channel 2 (Δ = 0.44 ns, p < 0.05) and channel 3 (Δ = 0.75 ns, p < 0.01) compared to control samples (channel 2: 6.34 ns; channel 3: 5.22 ns). Fluorescence intensity ratio values were lower in channel 1 (37%, p < 0.0001) and channel 2 (31% decrease, p < 0.0001) and higher in channel 3 (23%, p < 0.0001) relative to control samples. These results show that FLIm can detect the loss of GAG in articular cartilage and support further investigation into the feasibility of in vivo FLIm arthroscopy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.