ArticleJournal of biophotonics2019
Quantification of collagen fiber structure using second harmonic generation imaging and two-dimensional discrete Fourier transform analysis: Application to the human optic nerve head.
Article in Journal of biophotonics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 43 citations in OpenAlex.
- Segmentation-free Radon transform algorithm to detect orientation and size of tissue structures in multiphoton microscopy images.Journal of biomedical optics · 2025Article
- Learning-enhanced 3D fiber orientation mapping in thick cardiac tissues.Biomedical optics express · 2025Article
- What Is the Sequence of Mechanical and Structural Failure During Stretch Injury in the Rat Median Nerve? The Neuroclasis Classification.Clinical orthopaedics and related research · 2025Article
- Label-free multimodal optical biopsy reveals biomolecular and morphological features of diabetic kidney tissue in 2D and 3D.Nature communications · 2025Article
- Label-Free Optical Biopsy Reveals Biomolecular and Morphological Features of Diabetic Kidney Tissue in 2D and 3D.bioRxiv : the preprint server for biology · 2024Article
- Collagen organization and structure inBiomedical optics express · 2024Article
- Lamina Cribrosa Insertions Into the Sclera Are Sparser, Narrower, and More Slanted in the Anterior Lamina.Investigative ophthalmology & visual science · 2024Article
- Collagen organization and structure inbioRxiv : the preprint server for biology · 2024Article
- Fibrous finite element modeling of the optic nerve head region.Acta biomaterialia · 2024Article
- Three-dimensional cell culture conditions promoted the Mesenchymal-Amoeboid Transition in the Triple-Negative Breast Cancer cell line MDA-MB-231.Frontiers in cell and developmental biology · 2024Article
- A direct fiber approach to model sclera collagen architecture and biomechanics.Experimental eye research · 2023Article
- Who bears the load? IOP-induced collagen fiber recruitment over the corneoscleral shell.Experimental eye research · 2023Article
- Article
- Remodelling of the fibre-aggregate structure of collagen gels by cancer-associated fibroblasts: A time-resolved grey-tone image analysis based on stochastic modelling.Frontiers in immunology · 2022Article
- A quantitative analysis of bone lamellarity and bone collagen linearity induced by distinct dosing and frequencies of teriparatide administration in ovariectomized rats and monkeys.Microscopy (Oxford, England) · 2021Article
- Mechanosensitive channel inhibition attenuates TGFβ2-induced actin cytoskeletal remodeling and reactivity in mouse optic nerve head astrocytes.Experimental eye research · 2021Article
- Integrated Cells and Collagen Fibers Spatial Image Analysis.Frontiers in bioinformatics · 2021Article
- Decorin-An Antagonist of TGF-β in Astrocytes of the Optic Nerve.International journal of molecular sciences · 2021Article
- Spatial frequency metrics for analysis of microscopic images of musculoskeletal tissues.Connective tissue research · 2021Article
- The zonal evolution of collagen-network morphology quantified in early osteoarthritic grades of human cartilage.Osteoarthritis and cartilage open · 2020Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Second harmonic generation (SHG) microscopy is widely used to image collagen fiber microarchitecture due to its high spatial resolution, optical sectioning capabilities and relatively nondestructive sample preparation. Quantification of SHG images requires sensitive methods to capture fiber alignment. This article presents a two-dimensional discrete Fourier transform (DFT)-based method for collagen fiber structure analysis from SHG images. The method includes integrated periodicity plus smooth image decomposition for correction of DFT edge discontinuity artefact, avoiding the loss of peripheral image data encountered with more commonly used windowing methods. Outputted parameters are as follows: the collagen fiber orientation distribution, aligned collagen content and the degree of collagen fiber dispersion along the principal orientation. We demonstrate its application to determine collagen microstructure in the human optic nerve head, showing its capability to accurately capture characteristic structural features including radial fiber alignment in the innermost layers of the bounding sclera and a circumferential collagen ring in the mid-stromal tissue. Higher spatial resolution rendering of individual lamina cribrosa beams within the nerve head is also demonstrated. Validation of the method is provided in the form of correlative results from wide-angle X-ray scattering and application of the presented method to other fibrous tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.