ArticleJournal of clinical periodontology2019
Immunization with cell-free-generated vaccine protects from Porphyromonas gingivalis-induced alveolar bone loss.
Article in Journal of clinical periodontology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Advances in Periodontal Diagnostics: Application of MultiModal Language Models in Visual Interpretation of Panoramic Radiographs.Diagnostics (Basel, Switzerland) · 2025Article
- Exploring heme and iron acquisition strategies of Porphyromonas gingivalis-current facts and hypotheses.FEMS microbiology reviews · 2025Review
- Investigating oral microbiome profiles in patients with cleft lip and palate compared with the healthy control.BMC oral health · 2024Article
- Matrix Metalloproteinases in the Periodontium-Vital in Tissue Turnover and Unfortunate in Periodontitis.International journal of molecular sciences · 2024Review
- The PerioGene North study reveals that periodontal inflammation and advanced jawbone loss in periodontitis associate with serum gingipain antibodies but not with systemic autoimmunity.Frontiers in immunology · 2024Article
- Future Drug Targets in Periodontal Personalised Medicine-A Narrative Review.Journal of personalized medicine · 2022Review
- Reverse vaccinology approach to identify novel and immunogenic targets against Porphyromonas gingivalis: An in silico study.PloS one · 2022Article
- Computational Design of a Multi-Epitope Vaccine AgainstFrontiers in immunology · 2022Article
- AFrontiers in oral health · 2021Article
- Targeting a cysteine protease from a pathobiont alleviates experimental arthritis.Arthritis research & therapy · 2020Article
- Immunization with cell-free-generated vaccine protects from Porphyromonas gingivalis-induced alveolar bone loss.Journal of clinical periodontology · 2019Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionPeriodontal diseases (PD) are complex oral inflammatory diseases initiated by keystone bacteria such as Porphyromonas gingivalis. A vaccine for PD is desirable as clinical treatment involves protracted maintenance strategies aimed to retain dentition. Although prior immunization approaches targeting P. gingivalis have reported variable success in limiting facets of disease such as oral bone loss, it remains that a vaccine for this disease may be attainable.
aimTo investigate cell-free protein synthesis (CFPS) as a platform to produce vaccinable targets suitable for efficacy testing in a P. gingivalis-induced murine oral bone loss model. MATERIALS AND
methodsRecombinantly generated P. gingivalis minor fimbriae protein (Mfa1), RgpA gingipain hemagglutinin domain 1 (HA1), and RgpA gingipain hemagglutinin domain 2 (HA2) were combined in equivalent doses in adjuvants and injected intramuscularly to immunize mice. Serum levels of protein-specific antibody were measured by ELISA, and oral bone levels were defined by morphometrics.
resultsRecombinantly generated P. gingivalis proteins possessed high fidelity to predicted size and elicited protein-specific IgG following immunization. Importantly, immunization with the vaccine cocktail protected from P. gingivalis elicited oral bone loss.
conclusionThese data verify the utility of the CFPS technology to synthesize proteins that have the capacity to serve as novel vaccines.
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