Evidence map›Paper›PMID 30578276›Full record

ArticleDrug metabolism and disposition: the biological fate of chemicals2019

A Recombinant Humanized Anticocaine Monoclonal Antibody Alters the Urinary Clearance of Cocaine and Its Metabolites in Rats.

Jordan A Marckel, Hanna N Wetzel, Sihame Amlal, Hassane Amlal, Andrew B Norman

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Drug metabolism and disposition: the biological fate of chemicals, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.3field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Monoclonal Antibodies Counteract Opioid-Induced Behavioral and Toxic Effects in Mice and Rats.The Journal of pharmacology and experimental therapeutics · 2020
    Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Jordan A MarckelDepartments of Pharmacology and Systems Physiology (J.A.M., H.N.W., A.B.N.) and Internal Medicine, Division of Nephrology and Hypertension (S.A., H.A.), College of Medicine, University of Cincinnati, Cincinnati, Ohio andrew.norman@uc.edu.ORCID 0000-0002-3245-0968
Hanna N WetzelDepartments of Pharmacology and Systems Physiology (J.A.M., H.N.W., A.B.N.) and Internal Medicine, Division of Nephrology and Hypertension (S.A., H.A.), College of Medicine, University of Cincinnati, Cincinnati, Ohio.ORCID 0000-0002-1235-0759
Sihame AmlalDepartments of Pharmacology and Systems Physiology (J.A.M., H.N.W., A.B.N.) and Internal Medicine, Division of Nephrology and Hypertension (S.A., H.A.), College of Medicine, University of Cincinnati, Cincinnati, Ohio.
Hassane AmlalDepartments of Pharmacology and Systems Physiology (J.A.M., H.N.W., A.B.N.) and Internal Medicine, Division of Nephrology and Hypertension (S.A., H.A.), College of Medicine, University of Cincinnati, Cincinnati, Ohio.
Andrew B NormanDepartments of Pharmacology and Systems Physiology (J.A.M., H.N.W., A.B.N.) and Internal Medicine, Division of Nephrology and Hypertension (S.A., H.A.), College of Medicine, University of Cincinnati, Cincinnati, Ohio andrew.norman@uc.edu.
University of Cincinnati · US

Funding

Advancing the Development of a Humanized Anti-cocaine Monoclonal AntibodyU01DA039550 · NIDA · UNIVERSITY OF CINCINNATI · PI NORMAN, ANDREW B · 2015 to 2017
$6.3M
NIDA NIH HHS U01 DA039550
6 · The paper itself

Abstract

A recombinant humanized anticocaine monoclonal antibody, h2E2, has shown potential in the preclinical phases for the treatment of cocaine abuse. The standard tests for cocaine usage are the detection of benzoylecgonine (BE) and cocaine in the urine. This includes workplace drug screens as well as in clinical trials for potential treatments of cocaine abuse. By sequestering cocaine into the plasma compartment, h2E2 prevents cocaine from entering the brain. Due to the altered disposition of cocaine in the presence of h2E2, we investigated the effects of h2E2 on cocaine and metabolite levels in the urine of rats to clarify the use of BE as an endpoint measurement for effectiveness in future clinical trials. The urine concentrations of cocaine and metabolites were considerably altered in the presence of h2E2. After a single injection of h2E2 (120 mg/kg) and cocaine hydrochloride (0.56 mg/kg), the concentration of cocaine and BE excreted into the urine of rats decreased by 92% and 91%, respectively, from vehicle controls. Due to the significant decrease in urinary excretion, BE is not an appropriate indicator of cocaine usage in the presence of h2E2. Another endpoint measurement must be selected for the measurement of cocaine usage in the upcoming clinical trials of h2E2. In contrast to the effects on cocaine and BE urinary excretion, there was a 3-fold increase in ecgonine methyl ester (EME) in the presence of h2E2. Therefore, we conclude that EME is a more appropriate measurement of cocaine intake in the presence of h2E2.

Indexed as

AnimalsAntibodies, Monoclonal, HumanizedCocaineCocaine-Related DisordersDisease Models, AnimalHumansMaleRatsRats, Sprague-DawleyRecombinant ProteinsRenal EliminationSubstance Abuse DetectionAntibodies, Monoclonal, HumanizedbenzoylecgonineCocaineecgonine methyl esterRecombinant Proteins

Identifiers

PMID30578276
PMCPMC6367686
OpenAlexW2906672240

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.