ArticleMicrobial cell factories2018
Tuning recombinant protein expression to match secretion capacity.
Article in Microbial cell factories, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 45 citations in OpenAlex.
- High-cell-density Escherichia coli-based nanobody production: benchmarking of subcellular targeting, strain background and process development strategies.Microbial cell factories · 2026Article
- Construction of RecombinantMicroorganisms · 2025Article
- Data-driven learning of structure augments quantitative prediction of biological responses.PLoS computational biology · 2024Article
- Biosensor that Detects Stress Caused by Periplasmic Proteins.ACS synthetic biology · 2024Article
- Yields and product comparison between Escherichia coli BL21 and W3110 in industrially relevant conditions: anti-c-Met scFv as a case study.Microbial cell factories · 2023Article
- Precise Genomic Riboregulator Control of Metabolic Flux in Microbial Systems.ACS synthetic biology · 2022Article
- Strategies for efficient production of recombinant proteins in Escherichia coli: alleviating the host burden and enhancing protein activity.Microbial cell factories · 2022Review
- In silico and in vitro analysis of a rational mutation in gIII signal peptide and its effects on periplasmic expression of rhGH in E. coli.Archives of microbiology · 2022Article
- Developing of specific monoclonal recombinant antibody fused to alkaline phosphatase (AP) for one-step detection of fig mosaic virus.3 Biotech · 2022Article
- Genetic and process engineering strategies for enhanced recombinant N-glycoprotein production in bacteria.Microbial cell factories · 2021Article
- Strategies to Enhance Periplasmic Recombinant Protein Production Yields inFrontiers in bioengineering and biotechnology · 2021Review
- Efficient Secretion of Murine IL-2 From an Attenuated Strain ofFrontiers in microbiology · 2021Article
- Evolution ofInternational journal of molecular sciences · 2020Review
- A novel knock out strategy to enhance recombinant protein expression in Escherichia coli.Microbial cell factories · 2020Article
- Bacterial biopolymers: from pathogenesis to advanced materials.Nature reviews. Microbiology · 2020Review
- Extremely Low Leakage Expression Systems Using Dual Transcriptional-Translational Control for Toxic Protein Production.International journal of molecular sciences · 2020Review
- New tools for recombinant protein production in Escherichia coli: A 5-year update.Protein science : a publication of the Protein Society · 2019Review
- Systematic Evaluation of Genetic and Environmental Factors Affecting Performance of Translational Riboswitches.ACS synthetic biology · 2019Article
- Article
- Engineering a Microbial Consortium Based Whole-Cell System for Efficient Production of Glutarate From L-Lysine.Frontiers in microbiology · 2019Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe secretion of recombinant disulfide-bond containing proteins into the periplasm of Gram-negative bacterial hosts, such as E. coli, has many advantages that can facilitate product isolation, quality and activity. However, the secretion machinery of E. coli has a limited capacity and can become overloaded, leading to cytoplasmic retention of product; which can negatively impact cell viability and biomass accumulation. Fine control over recombinant gene expression offers the potential to avoid this overload by matching expression levels to the host secretion capacity.
resultsHere we report the application of the RiboTite gene expression control system to achieve this by finely controlling cellular expression levels. The level of control afforded by this system allows cell viability to be maintained, permitting production of high-quality, active product with enhanced volumetric titres.
conclusionsThe methods and systems reported expand the tools available for the production of disulfide-bond containing proteins, including antibody fragments, in bacterial hosts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.